Recombinant SARS-CoV-2 Envelope (E) Protein

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SKU:
CARP01263
Product Type:
Antibody
Antibody Type:
Monoclonal Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG

Description

Technical Manual

SARS-CoV-2 Envelope Protein - Background Information

The envelope protein is one of the four integral proteins found on SARS-CoV-2 viral particles. The protein has a central role in virus morphogenesis and assembly, acting as a viroporin and self-assembling in host membranes to form pentameric protein-lipid pores that allow ion transport. The E protein also plays a role in the induction of apoptosis (by similarity).

SARS-CoV-2 Envelope Protein Properties

Description Recombinant 2019-nCoV envelope Protein is produced by E. coli expression system. The target protein is expressed with sequence (Met1-Val75) of 2019-ncov envelope (Accession #QHD43418.1) fused with a 6×His, Avi tag at the N-terminus.
Size 100μg
Expressed Host E. coli
Species SARS-CoV-2
Application Western blot, immunofluorescence, immunohistochemistry, immunoprecipitation
Purity >92% by SDS-PAGE.
Formulation Supplied as a 0.22 μm filtered solution in 20mM Tris, 250mM NaCl, 0.5%TritonX-100, pH 8.0.
Tag 6×His and Avi tag at the N-terminus.
Gene ID 43740570
Endotoxin < 1.0 EU/μg of the protein by LAL method.
Storage This product is stable at ≤‑70°C for up to 6 months from the date of receipt. For optimal storage, aliquot into smaller quantities after centrifugation and store at recommended temperature. Avoid repeated freeze-thaw cycles.
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