Description
| Product Name: | Reactive Oxygen Species (ROS) Assay Kit - Fluorometric |
| Product Code: | MAES0112 |
| Product Size: | 96 Assays |
| Sample Type: | Fresh tissue, cultured cells |
| Assay Time: | 60 min |
| Instrument: | Fluorescence Microplate reader, Fluorescence Microscope, Flow Cytometry |
Reactive oxygen species (ROS) are active chemical substances produced in the metabolic process of the body, including oxygen free radicals, hydrogen peroxide and its downstream products, such as peroxides and hydroxides. ROS are both necessary and harmful to organisms, and are involved in cell growth, proliferation, development and differentiation, aging and apoptosis, as well as many physiological and pathological processes. Excessive ROS will lead to oxidative stress and oxidative damage of cells, further promoting the development of many diseases, such as cancer, cardiovascular disease and diabetes.
DCFH-DA (2,7-dichlorofuorescin diacetate) is a fluorescent probe without fluorescence that can freely cross the membrane. After entering the cell, it can be hydrolyzed by intracellular esterase to form DCFH (dichlorofluorescin). In the presence of reactive oxygen species (ROS), DCFH is oxidized to DCF (dichlorofluorescein) which is a strong green fluorescent substance that cannot penetrate the cell membrane. DCF has a maximum wave peak near the excitation wavelength of 502 nm and the emission wavelength of 525 nm, and the intensity is proportional to the level of intracellular reactive oxygen species.
| Shelf Life: | 6 Months |
| Storage: | Reagent 1:-20°C;, Others:2-8°C |
| Shipping: | Gel Pack |
| Research Area: | Oxidative stress |
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