RCN3 Antibody (PACO60312)
- SKU:
- PACO60312
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Applications:
- ELISA
- Applications:
- WB
- Applications:
- IHC
- Antibody Type:
- Polyclonal Antibody
- Conjugation:
- Unconjugated
Description
| Antibody Name: | RCN3 Antibody (PACO60312) |
| Antibody SKU: | PACO60312 |
| Size: | 50ug |
| Host Species: | Rabbit |
| Tested Applications: | ELISA, WB, IHC |
| Recommended Dilutions: | ELISA:1:2000-1:10000, WB:1:500-1:5000, IHC:1:200-1:500 |
| Species Reactivity: | Human, Mouse |
| Immunogen: | Recombinant Human Reticulocalbin-3 protein (71-161AA) |
| Form: | Liquid |
| Storage Buffer: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
| Purification Method: | >95%, Protein G purified |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Conjugate: | Non-conjugated |
 | IHC image of PACO60312 diluted at 1:200 and staining in paraffin-embedded human liver tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
 | Western Blot. Positive WB detected in: Hela whole cell lysate, U251 whole cell lysate, Mouse heart tissue. All lanes: RCN3 antibody at 4.6µg/ml. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 38 kDa. Observed band size: 38 kDa. |
 | IHC image of PACO60312 diluted at 1:200 and staining in paraffin-embedded human adrenal gland tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
| Synonyms: | Reticulocalbin-3 (EF-hand calcium-binding protein RLP49), RCN3 |
| UniProt Protein Function: | RCN3: Belongs to the CREC family.Protein type: Secreted; Secreted, signal peptideChromosomal Location of Human Ortholog: 19q13.33Cellular Component: endoplasmic reticulum lumenMolecular Function: protein binding; calcium ion binding |
| UniProt Protein Details: | |
| NCBI Summary: | |
| UniProt Code: | Q96D15 |
| NCBI GenInfo Identifier: | 28626510 |
| NCBI Gene ID: | 57333 |
| NCBI Accession: | NP_065701.2 |
| UniProt Secondary Accession: | Q96D15,Q9HBZ8 |
| UniProt Related Accession: | Q96D15 |
| Molecular Weight: | 37,493 Da |
| NCBI Full Name: | reticulocalbin-3 |
| NCBI Synonym Full Names: | reticulocalbin 3, EF-hand calcium binding domain |
| NCBI Official Symbol: | RCN3 |
| NCBI Official Synonym Symbols: | RLP49 |
| NCBI Protein Information: | reticulocalbin-3; reticulocabin; EF-hand calcium-binding protein RLP49 |
| UniProt Protein Name: | Reticulocalbin-3 |
| UniProt Synonym Protein Names: | EF-hand calcium-binding protein RLP49 |
| Protein Family: | Reticulocalbin |
| UniProt Gene Name: | RCN3 |
| UniProt Entry Name: | RCN3_HUMAN |
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