Description
Antibody Name: | rbsK Antibody (PACO33956) |
Antibody SKU: | PACO33956 |
Size: | 50ug |
Host Species: | Rabbit |
Tested Applications: | ELISA, WB |
Recommended Dilutions: | ELISA:1:2000-1:10000, WB:1:500-1:5000 |
Species Reactivity: | Escherichia coli |
Immunogen: | Recombinant Escherichia coli Ribokinase protein (1-309AA) |
Form: | Liquid |
Storage Buffer: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
Purification Method: | >95%, Protein G purified |
Clonality: | Polyclonal |
Isotype: | IgG |
Conjugate: | Non-conjugated |
![]() | Western blot All lanes: rbsK antibody at 2µg/ml + DH5a whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 33 kDa Observed band size: 33 kDa . |
![]() | Western Blot Positive WB detected in: Rosseta bacteria lysate at 80μg, 40μg, 20μg, 10μg All lanes: rbsK antibody at 3µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 33 kDa Observed band size: 33 kDa . |
Background: | In enzymology, a ribokinase (EC 2.7.1.15) is an enzyme that catalyzes the chemical reaction nATP + D-ribose ADP + D-ribose 5-phosphate.Thus, the two substrates of this enzyme are ATP and D-ribose, whereas its two products are ADP and D-ribose 5-phosphate.This enzyme belongs to the family of transferases, specifically those transferring phosphorus-containing groups (phosphotransferases) with an alcohol group as acceptor. The systematic name of this enzyme class is ATP:D-ribose 5-phosphotransferase. Other names in common use include deoxyribokinase, ribokinase (phosphorylating), and D-ribokinase. This enzyme participates in pentose phosphate pathway. |
Synonyms: | Ribokinase (RK) (EC 2.7.1.15), rbsK |
UniProt Protein Function: | Catalyzes the phosphorylation of ribose at O-5 in a reaction requiring ATP and magnesium. The resulting D-ribose-5-phosphate can then be used either for sythesis of nucleotides, histidine, and tryptophan, or as a component of the pentose phosphate pathway. |
UniProt Protein Details: | |
NCBI Summary: | |
UniProt Code: | P0A9J6 |
NCBI GenInfo Identifier: | 16131620 |
NCBI Gene ID: | 948260 |
NCBI Accession: | NP_418208.1 |
UniProt Secondary Accession: | P0A9J6,P05054, Q2M870 |
UniProt Related Accession: | P0A9J6 |
Molecular Weight: | 32kd |
NCBI Full Name: | ribokinase |
NCBI Synonym Full Names: | |
NCBI Official Symbol: | rbsK |
NCBI Official Synonym Symbols: | ECK3746; JW3731 |
NCBI Protein Information: | ribokinase |
UniProt Protein Name: | Ribokinase |
UniProt Synonym Protein Names: | |
Protein Family: | Ribokinase |
UniProt Gene Name: | rbsK |
UniProt Entry Name: | RBSK_ECOLI |