Rat Immunology ELISA Kits 3

Rat TSH ELISA Kit

(No reviews yet) Write a Review
SKU:
RTFI01195
Product Type:
ELISA Kit
Size:
96 Assays
Sensitivity:
0.75ng/ml
Range:
1.25-80ng/ml
ELISA Type:
Competitive
Synonyms:
TSH, Thyroid Stimulating Hormone
Reactivity:
Rat

Description

Rat TSH ELISA Kit

The Rat TSH ELISA kit is designed for the quantitative measurement of rat Thyroid Stimulating Hormone (TSH) levels in various biological samples. TSH is a crucial hormone that regulates the thyroid gland's function and plays a pivotal role in maintaining metabolic balance. This kit enables researchers to accurately assess TSH concentrations in rat samples, aiding investigations into thyroid function, endocrine disorders, and related research areas.

system_update_alt Datasheet system_update_alt MSDS

Key Features

Save Time Pre-coated 96 well plate
Quick Start Kit includes all necessary reagents
Publication Ready Reproducible and reliable results

Overview

Product Name:

Rat TSH (Thyroid Stimulating Hormone) ELISA Kit

Product Code:

RTFI01195

Size:

96 Assays

Alias:

TSH, Thyroid Stimulating Hormone

Detection Method:

Competitive ELISA, Coated with Antibody

Reactivity:

Rat

Sensitivity:

0.75ng/ml

Range:

1.25-80ng/ml

Storage:

4°C for 6 months

Note:

For Research Use Only

Additional Information

Recovery

Matrices listed below were spiked with certain level of Mouse TSH and the recovery rates were calculated by comparing the measured value to the expected amount of Mouse TSH in samples.

Matrix

Recovery Range (%)

Average (%)

serum (n=5)

88-101

94

EDTA plasma (n=5)

89-103

97

UFH plasma (n=5)

89-103

96

Linearity:

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Mouse TSH and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample

1:2

1:4

1:8

Serum (n=5)

87-100%

89-104%

87-102%

EDTA plasma (n=5)

87-95%

85-98%

85-99%

UFH Plasma (n=5)

83-97%

81-92%

80-100%

Intra Assay

CV < 8%

Inter Assay

CV < 10%

Kit Components

Component Quantity Storage

ELISA Microplate (Dismountable)

8x12 strips

4°C for 6 months

Lyophilized Standard

2

4°C/ -20°C

Sample/Standard Dlution Buffer

20ml

4°C

Biotin-labeled Antibody (Concentrated)

120ul

4°C (Protection from light)

Antibody Dilution Buffer

10ml

4°C

HRP-Streptavidin Conjugate (SABC)

120ul

4°C (Protect from light)

SABC Dilution Buffer

10ml

4°C

TMB Substrate

10ml

4°C (Protection from light)

Stop Solution

10ml

4°C

Wash Buffer (25X)

30ml

4°C

Plate Sealer

View AllClose

0 Reviews

View AllClose