Rat PTH PharmaGenie ELISA Kit (SBRS0156)

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SKU:
SBRS0156
Product Type:
ELISA Kit
Reactivity:
Rat
Applications:
ELISA
ELISA Type:
Competitive

Description

Rat PTH PharmaGenie ELISA - Kit Information

Parathyroid hormone (PTH) is secreted by the chief cells of the parathyroid glands as a polypeptide containing 84 amino acids with molecular weight of 9.4KDa. PTH acts to increase the concentration of calcium (Ca2+) in the blood by acting upon parathyroid hormone receptor in three parts of the body: bone, kidney and intestine. In addition to regulation of serum calcium levels, PTH also plays role in regulating serum phosphate and vitamin D synthesis. PTH reduces the reabsorption of phosphate from the proximal tubule of the kidney, resulting in increased phosphate excretion through the urine. However, PTH enhances the uptake of phosphate from the intestine and bones into the blood. In the bone, slightly more calcium than phosphate is released from the breakdown of bone. In the intestines, which are mediated by an increase in activated vitamin D, the absorption of phosphate is not as dependent on vitamin D as is that of calcium. The end result is a small net drop in the serum concentration of phosphate. PTH increases the activity of 1-alpha-hydroxylase enzyme, which converts 25-hydroxycholecalciferol to 1,25-dihydroxycholecalciferol, the active form of vitamin D. PTH has shown significant clinical applications. A high level of PTH in the blood is known as hyperparathyroidism. The causes of primary hyperparathyroidism are parathyroid adenoma, parathyroid hyperplasia and parathyroid cancer. The cause for secondary hyperparathyroidism is often due to in chronic renal failure.

Rat PTH PharmaGenie ELISA - Kit Data

Product SKU:SBRS0156
Size:96T
Application:Cross Reactivity: This ELISA kit shows no cross-reactivity with any of the cytokines tested: Ghrelin, Nesfatin, Angiotensin II, NPY and APC.
Uniprot:P04089
Gene ID:24694
Gene Names:PTH
Synonyms:Parathyroid hormone (PTH) (Parathyrin)
Target Species:Human, Mouse, Rat
Compatible Sample Types:Cell Culture Supernatants, Serum
Design Principle:Competition-based
Method of Detection:Colorimetric
Quantitative/Semi-Quantitative:Quantitative
Range:1-1,000 pg/ml
Sensitivity:1.27 pg/ml
Recommended Dilution:Human: 2X, Mouse: 2X, Rat: 2X
Storage/Stability:Standard, Biotinylated Parathyroid Hormone (PTH) peptide, and Positive Control should be stored at -20°C after arrival. Avoid multiple freeze-thaws. The remaining kit components may be stored at 4°C. Opened Microplate Wells and antibody (Item N) may be stored for up to 1 month at 2 to 8°C. Return unused wells to the pouch containing desiccant pack and reseal along entire edge.

Kit Components:

1.Pre-Coated 96-well Strip Microplate
2.Wash Buffer
3.Standard Peptide
4.Assay Diluent(s)
5.Biotinylated Peptide
6.HRP-Streptavidin
7.TMB One-Step Substrate
8.Stop Solution
9.Assay Diagram
10.Positive Control Sample
11.Capture Antibody
12.Technical Manual

Other materials and equipment required:

The Assay Genie Rat PTH ELISA Kit will require other equipment and materials to carry out the assay. Please see list below for further details.

  • Distilled or deionized water
  • Precision pipettes to deliver 2 ul to 1 ml volumes
  • Adjustable 1-25 ml pipettes for reagent preparation
  • 100 ml and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Orbital shaker
  • Aluminum foil
  • Saran Wrap
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • SigmaPlot software (or other software that can perform four-parameter logistic regression models)

Kit Protocol:

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