Description
| Product Name: | Rat PFN1 Recombinant Protein |
| Product Code: | RPPB4234 |
| Size: | 20µg |
| Species: | Rat |
| Target: | PFN1 |
| Synonyms: | Profilin-1, Profilin I. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered colorless solution. |
| Formulation: | PFN1 protein solution (1mg/ml) containing PhosphateBuffered Saline (pH 7.4) and 10% glycerol 1mM DTT. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 95.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MGSHMAGWNA YIDSLMADGT CQDAAIVGYK DSPSVWAAVP GKTFVSITPAEVGVLVGKDR SSFFVNGLTL GGQKCSVIRD SLLQDGEFTM DLRTKSTGGA PTFNVTVTMT AKTLVLLMGKEGVHGGLINK KCYEMASHLR RSQY |
Profilin-1 also known as Pfn1 is a ubiquitous actin monomer-bindingprotein which is a member of the profilin family. Pfn1 significantly enhancesskin wound healing in-vitro as well as in-vivo which is mediated by purinergicreceptors. Furthermore, Pfn1 is also active in endothelial cell migration andvessel sprouting. Pfn1 is considered to regulate actin polymerization inresponse to extracellular signals.
PFN1 Rat Recombinant produced in E.Coli is a single,non-glycosylated polypeptide chain containing 164 amino acids (1-140 a.a) andhaving a molecular mass of 17.5kDa.PFN1 is fused to a 24 amino acid His-tag at N-terminus& purified by proprietary chromatographic techniques.
| UniProt Protein Function: | profilin 1: a ubiquitous actin monomer-binding protein belonging to the profilin family. At high concentrations, profilin 1 prevents the polymerization of actin, whereas it enhances it at low concentrations. By binding to PIP2, it inhibits the formation of IP3 and DG. Deletion of this gene is associated with Miller-Dieker syndrome. Protein type: Motility/polarity/chemotaxis; Cytoskeletal; Actin-bindingCellular Component: neuron projection; focal adhesion; cytoskeleton; membrane; cytoplasm; synapse; nucleusMolecular Function: protein binding; phosphatidylinositol-4,5-bisphosphate binding; Rho GTPase binding; adenyl-nucleotide exchange factor activity; actin binding; receptor bindingBiological Process: positive regulation of DNA metabolic process; negative regulation of actin filament bundle formation; negative regulation of stress fiber formation; negative regulation of actin filament polymerization; positive regulation of viral transcription; regulation of transcription from RNA polymerase II promoter; response to organic substance; positive regulation of actin filament polymerization; positive regulation of ATPase activity; actin polymerization and/or depolymerization; positive regulation of stress fiber formation; neural tube closure; positive regulation of transcription from RNA polymerase II promoter |
| UniProt Protein Details: | |
| NCBI Summary: | regulates actin polymerization; may reorganize the actin cytoskeleton; may be involved in the pathogenesis of glomerulonephritis [RGD, Feb 2006] |
| UniProt Code: | P62963 |
| NCBI GenInfo Identifier: | 42476144 |
| NCBI Gene ID: | 64303 |
| NCBI Accession: | NP_071956.2 |
| UniProt Secondary Accession: | P62963,P10924 |
| UniProt Related Accession: | P62963 |
| Molecular Weight: | 14,957 Da |
| NCBI Full Name: | profilin-1 |
| NCBI Synonym Full Names: | profilin 1 |
| NCBI Official Symbol: | Pfn1�� |
| NCBI Official Synonym Symbols: | |
| NCBI Protein Information: | profilin-1 |
| UniProt Protein Name: | Profilin-1 |
| UniProt Synonym Protein Names: | Profilin I |
| Protein Family: | Profilin |
| UniProt Gene Name: | Pfn1�� |
| UniProt Entry Name: | PROF1_RAT |
