Description
| Product Name: | Rat PDGF BB Recombinant Protein |
| Product Code: | RPPB0841 |
| Size: | 10µg |
| Species: | Rat |
| Target: | PDGF BB |
| Synonyms: | Platelet-derived growth factor subunit B, PDGF subunit B, PDGF-2, Platelet-derived growth factor B chain, Platelet-derived growth factor beta polypeptide, Pdgfb, SIS, c-sis. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation: | PDGF-BB was lyophilized from a 0.2�m filtered concentrated solution in 1xPBS, pH 7.0. |
| Solubility: | It is recommended to reconstitute the lyophilized PDGF-BB in sterile 18M-cm H2O not less than 100�g/ml, which can then be further diluted to other aqueous solutions. |
| Stability: | Lyophilized Platelet-derived Growth Factor BB although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution PDGF BB should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles. |
| Purity: | Greater than 98.0% as determined by:(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE. |
| Amino Acid Sequence: | SLGSLAAAEP AVIAECKTRT EVFQISRNLI DRTNANFLVW PPCVEVQRCS GCCNNRNVQC RASQVQMRPV QVRKIEIVRK KPVFKKATVT LEDHLACKCE TVVTPRPVT |
| Biological Activity: | The ED50as determined by a cell proliferation assay using murine Balb/c 3T3 cells is less than 2.0 ng/ml, corresponding to a specific activity of > 5.0 � 105 IU/mg. |
PDGF-BB is a member of the platelet-derived growth factor family. The four members of this family are mitogenic factors for cells of mesenchymal origin and are characterized by a motif of eight cysteines. This gene product can exist either as a homodimer (PDGF-BB) or as a heterodimer with the platelet-derived growth factor alpha polypeptide (PDGF-AB), where the dimers are connected by disulfide bonds. Mutations in this gene are associated with meningioma. Reciprocal translocations between chromosomes 22 and 7, at sites where this gene and that for COL1A1 are located, are associated with a particular type of skin tumor called dermatofibrosarcoma protuberans resulting from unregulated expression of growth factor. Two splice variants have been identified for this gene.
PDGF-BB Rat Recombinant produced in E.coli is a disulfide-linked homodimeric, non-glycosylated, polypeptide of two B chains containing 2x109 amino acids (218 amino acids in total) and having a molecular mass of 24.4 kDa.The PDGF-BB is purified by proprietary chromatographic techniques.
| UniProt Protein Function: | PDGFB: Growth factor that plays an essential role in the regulation of embryonic development, cell proliferation, cell migration, survival and chemotaxis. Potent mitogen for cells of mesenchymal origin. Required for normal proliferation and recruitment of pericytes and vascular smooth muscle cells in the central nervous system, skin, lung, heart and placenta. Required for normal blood vessel development, and for normal development of kidney glomeruli. Plays an important role in wound healing. Signaling is modulated by the formation of heterodimers with PDGFA. A chromosomal aberration involving PDGFB is found in dermatofibrosarcoma protuberans. Translocation t(17;22)(q22;q13) with PDGFB. Belongs to the PDGF/VEGF growth factor family. |
| UniProt Protein Details: | Protein type:Secreted; Oncoprotein; Secreted, signal peptide; Motility/polarity/chemotaxis Cellular Component: basolateral plasma membrane; cell soma; cell surface; cytoplasm; dendrite; extracellular space; intracellular; membrane Molecular Function:chemoattractant activity; collagen binding; growth factor activity; identical protein binding; platelet-derived growth factor binding; platelet-derived growth factor receptor binding; protein heterodimerization activity; protein homodimerization activity; receptor binding; superoxide-generating NADPH oxidase activator activity Biological Process: actin cytoskeleton organization and biogenesis; activation of protein kinase activity; activation of protein kinase B; blood vessel morphogenesis; cell growth; cell projection biogenesis; DNA replication; embryonic placenta development; eye photoreceptor cell development; glial cell development; heart development; hemopoiesis; monocyte chemotaxis; negative regulation of cell migration; negative regulation of phosphatidylinositol biosynthetic process; negative regulation of protein binding; negative regulation of transcription, DNA-dependent; neuron remodeling; peptidyl-serine phosphorylation; peptidyl-tyrosine phosphorylation; platelet-derived growth factor receptor signaling pathway; positive chemotaxis; positive regulation of blood vessel endothelial cell migration; positive regulation of cell migration; positive regulation of cell proliferation; positive regulation of chemotaxis; positive regulation of collagen biosynthetic process; positive regulation of cyclin-dependent protein kinase activity; positive regulation of DNA replication; positive regulation of endothelial cell proliferation; positive regulation of fibroblast growth factor receptor signaling pathway; positive regulation of fibroblast proliferation; positive regulation of glomerular filtration; positive regulation of MAP kinase activity; positive regulation of MAPKKK cascade; positive regulation of mitosis; positive regulation of mitotic cell cycle, embryonic; positive regulation of peptidyl-tyrosine phosphorylation; positive regulation of phosphoinositide 3-kinase activity; positive regulation of phosphoinositide 3-kinase cascade; positive regulation of protein amino acid autophosphorylation; positive regulation of smooth muscle cell migration; positive regulation of smooth muscle cell proliferation; positive regulation of transcription, DNA-dependent; protein amino acid phosphorylation; regulation of cell proliferation; regulation of peptidyl-tyrosine phosphorylation; response to axon injury; response to drug; response to estradiol stimulus; response to estrogen stimulus; response to hypoxia; response to insulin stimulus; response to organic cyclic substance; response to organic substance; response to wounding; retina development in camera-type eye; substrate-bound cell migration; synaptogenesis; wound healing |
| NCBI Summary: | may play a role in carotid artery smooth muscle cell proliferation and neointimal formation in response to balloon catheter injury [RGD, Feb 2006] |
| UniProt Code: | Q05028 |
| NCBI GenInfo Identifier: | 158081747 |
| NCBI Gene ID: | 24628 |
| NCBI Accession: | NP_113712.1 |
| UniProt Related Accession: | Q05028 |
| Molecular Weight: | 25,603 Da |
| NCBI Full Name: | platelet-derived growth factor subunit B |
| NCBI Synonym Full Names: | platelet derived growth factor subunit B |
| NCBI Official Symbol: | Pdgfb�� |
| NCBI Official Synonym Symbols: | SIS; c-sis�� |
| NCBI Protein Information: | platelet-derived growth factor subunit B |
| UniProt Protein Name: | Platelet-derived growth factor subunit B |
| UniProt Synonym Protein Names: | PDGF-2; Platelet-derived growth factor B chain; Platelet-derived growth factor beta polypeptide |
| Protein Family: | Platelet-derived growth factor |
| UniProt Gene Name: | Pdgfb�� |
| UniProt Entry Name: | PDGFB_RAT |
