Enzymes Recombinant Proteins

Rat LDHA Recombinant Protein (RPPB1895)

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SKU:
RPPB1895
Product Type:
Recombinant Protein
Species:
Rat
Uniprot:
P04642
Research Area:
Enzymes

Description

system_update_altDatasheetsystem_update_altMSDS
Product Name:Rat LDHA Recombinant Protein
Product Code:RPPB1895
Size:10µg
Species:Rat
Target:LDHA
Synonyms:L-lactate dehydrogenase A chain, LDH-A, LDH musclesubunit, LDH-M.
Source:Sf9 Insect cells
Physical Appearance:Sterile Filtered colorless solution.
Formulation:LDHA protein solution (0.25mg/ml) containing PhosphateBuffered Saline (pH 7.4), 1mM DTT and 20% glycerol.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.�For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).�Avoid multiple freeze-thaw cycles.
Purity:Greaterthan 95.0% as determined by SDS-PAGE.
Amino Acid Sequence:MAALKDQLIV NLLKEEQVPQ NKITVVGVGA VGMACAISIL MKDLADELALVDVIEDKLKG EMMDLQHGSL FLKTPKIVSS KDYSVTANSK LVIITAGARQ QEGESRLNLV QRNVNIFKFIIPNVVKYSPQ CKLLIVSNPV DILTYVAWKI SGFPKNRVIG SGCNLDSARF RYLMGERLGV HPLSCHGWVLGEHGDSSVPV WSGVNVAGVS LKSLNPQLGT DADKEQWKDV HKQVVDSAYE VIKLKGYTSW AIGLSVADLAESIMKNLRRV HPISTMIKGL YGIKEDVFLS VPCILGQNGI SDVVKVTLTP DEEARLKKSA DTLWGIQKEL QFLEHHHHHH

LDHA catalyzes the conversion of L-lactate and NAD to pyruvate and NADH in the final step of anaerobic glycolysis. LDHA is localized primarily in muscle tissue and is part of the lactate dehydrogenase family. Mutations in LDHA have been linked to exertional myoglobinuria. LDH1 is decreased in essential thrombocythemia. LDHA is induced through a non-genomic pathway of estrogen action. Reduction in LDH-A activity results in stimulation of mitochondrial respiration and decrease of mitochondrial membrane potential.

LDHA Rat Recombinant produced in Sf9 Baculovirus cells isa single, glycosylated polypeptide chain containing 340 amino acids (1-332 a.a)and having a molecular mass of 37.5kDa (Migrates at 28-40kDa on SDS-PAGE underreducing conditions).�LDHA is fused to an 8 amino acid His-tag at C-terminus& purified by proprietary chromatographic techniques.

NCBI Summary:mRNA expression in fibroblasts increases in response to induction by epidermal growth factor or serum [RGD, Feb 2006]
UniProt Code:P04642
NCBI GenInfo Identifier:8393706
NCBI Gene ID:24533
NCBI Accession:NP_058721
UniProt Related Accession:P04642
Molecular Weight:37.5kDa (340aa) 28-40KDa (SDS-PAGE under reducing conditions)
NCBI Full Name:L-lactate dehydrogenase A chain
NCBI Synonym Full Names:lactate dehydrogenase A
NCBI Official Symbol:Ldha��
NCBI Official Synonym Symbols:CDK1; Ldh1��
NCBI Protein Information:L-lactate dehydrogenase A chain
UniProt Protein Name:L-lactate dehydrogenase A chain
UniProt Synonym Protein Names:LDH muscle subunit; LDH-M
Protein Family:L-lactate dehydrogenase
UniProt Gene Name:Ldha��
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