Rat Immune Checkpoint Molecule Array (9 targets) (SARB0104)

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SKU:
SARB0104
Product Type:
Protein Array
Reactivity:
Rat
Applications:
Multiplex Array

Description

system_update_altDatasheet
Product Sku: SARB0104
Size: 2, 4, or 8
Species Detected: Rat
Number of Targets Detected: 9
Gene Symbols: CD27, CD80, CD86, FAS, FASLG, HAVCR1, LGALS1, LGALS3, TNFRSF9
Compatible Sample Types: Cell Culture Supernatants, Cell Lysates, Plasma, Serum, Tissue Lysates
Design Principle: Sandwich-based
Method of Detection: Chemiluminescence
Quantitative/Semi-Quantitative: Semi-Quantitative
Solid Support: Membrane
Suggested Application(s): Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process; Detecting Nephrotoxicity
Storage/Stability: For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
Hover over target for more information
4-1BB(TNFRSF9/CD137)
  • Gene Symbol: Tnfrsf9 4-1BB LOC500590
  • Accession #: Q4W8J3
 CD27(TNFRSF7)
  • Gene Symbol: Cd27 MGC112688 rCG_30251
  • Gene ID: 500318
  • Accession #: Q501W2
 CD80(B7-1)
  • Gene Symbol: Cd80 B7-1
  • Accession #: Q62680
 CD86(B7-2)
  • Gene Symbol:Cd86 rCG_52586
  • Gene ID: 56822
  • Accession #: O35531
 Galectin-1
  • Gene Symbol: Lgals1
  • Gene ID: 56646
  • Accession #: P11762
Galectin-3
  • Gene Symbol: Lgals3
  • Gene ID: 83781
  • Accession #: P08699
 Fas(TNFRSF6/Apo-1)
  • Gene Symbol: Fas Apt1 Tnfrsf6
  • Gene ID: 246097
  • Accession #: Q63199
 Fas Ligand(TNFSF6)
  • Gene Symbol: Faslg Apt1Lg1 Cd95l Fasl Tnfsf6
  • Gene ID: 25385
  • Accession #: P36940
 TIM-1(KIM-1)
  • Gene Symbol: Havcr1 Kim1
  • Gene ID: 286934
  • Accession #: O54947
  • Easy to use
  • No specialized equipment needed
  • Compatible with nearly any liquid sample
  • Proven technology
  • Highly sensitive (pg/ml)
  • Sandwich ELISA specificity
  • Higher density than ELISA, Western blot or bead-based multiplex
  • Rat Immune Checkpoint Array
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated HRP
  • Detection Buffer C
  • Detection Buffer D
  • Lysis Buffer
  • 8-Well Incubation Tray
  • Plastic Sheets
  • Array Templates

    • Other Materials Required

    • Pipettors, pipet tips and other common lab consumables
    • Orbital shaker or oscillating rocker
    • Tissue Paper, blotting paper or chromatography paper
    • Adhesive tape or Saran Wrap
    • Distilled or de-ionized water
    • A chemiluminescent blot documentation system , X-ray Film and a suitable film processor, or another chemiluminescent detection system.
      1. Block membranes
      2. Incubate with Sample
      3. Incubate with Biotinylated Detection Antibody Cocktail
      4. Incubate with HRP-Conjugated Streptavidin
      5. Incubate with Detection Buffers
      6. Image with chemiluminescent imaging system
      7. Perform densitometry and analysis
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