Rat IL1R1/CD121a Recombinant Protein (RPES1382)
- SKU:
- RPES1382
- Product Type:
- Recombinant Protein
- Species:
- Rat
Description
Rat IL1R1/CD121a Recombinant Protein
Interleukin 1 receptor, type I (IL-1R1) also known as CD121a (Cluster of Differentiation 121a), is an interleukin receptor. IL-1R1/CD121a is a cytokine receptor that belongs to the interleukin 1 receptor family. This protein is a receptor for interleukin alpha (IL1A), interleukin beta (IL1B), and interleukin 1 receptor, type I (IL1R1/IL1RA). IL-1R1/CD121a is an important mediator involved in many cytokine induced immune and inflammatory responses. This protein has been characterized by pharmacological and molecular techniques in the mouse brain. The spindle-shaped astrocytes enclose the wound, separating the healthy from damaged neural tissue. The shape change and subsequent repair processes are IL-1β activity-dependent, acting through the IL-1 type 1 receptor (IL-1R1), as co-application of the IL-1type 1 receptor antagonist protein (IL-1ra) blocks IL-1β induced effects. In the spleen, a slight increase in IL-1R AcP and IL-1R1 was observed during the first hours following LPS stimulation. In conclusion, IL-1R AcP mRNA is expressed in the brain and in other tissues where IL-1R1/CD121a transcripts are found. However, the regulation of its expression is distinct from IL-1R1/CD121a. The high level of expression and the lack of regulation of IL-1R AcP transcripts in the brain under inflammatory conditions suggest that the protein might be constitutively expressed in excess.
| Product Name: | Rat IL1R1/CD121a Recombinant Protein (RPES1382) |
| Product Code: | RPES1382 |
| Size: | 50µg |
| Species: | Rat |
| Expressed Host: | HEK293 Cells |
| Synonyms: | Il1r1 |
| Accession: | NP_037255.3 |
| Sequence: | Met 1-Lys 352 |
| Fusion tag: | C-His-Fc |
| Activity: | Measured by its ability to inhibit IL1?-dependent proliferation in D10.G4.1 mouse helper T cells?Symons, J.A. et al. (1987) in Lymphokines and Interferons, a Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 272.).The ED50 for this effect is typically 5-20 µg/ml in the presence of 40 pg/mL of recombinant human IL1a. |
| Endotoxin: | <1.0 EU per µg of the protein as determined by the LAL method |
| Protein Construction: | A DNA sequence encoding the rat IL1R1 (NP_037255.3) extracellular domain (Met 1-Lys 352) was was fused with the C-terminal His-tagged Fc region of mouse IgG1 at the C-terminus. |
| Purity: | > 85 % as determined by SDS-PAGE |
| Mol Mass: | 64.6 kDa |
| AP Mol Mass: | 90-95 kDa |
| Formulation: | Lyophilized from sterile PBS, pH 7.4 |
| Shipping: | This product is provided as lyophilized powder which is shipped with ice packs. |
| Stability and Storage: | Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months. |
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