Cytokines Recombinant Proteins
Rat IL 4 Recombinant Protein (RPPB0509)
- SKU:
- RPPB0509
- Product Type:
- Recombinant Protein
- Species:
- Rat
- Uniprot:
- P20096
- Research Area:
- Cytokines
Description
| Product Name: | Rat IL 4 Recombinant Protein |
| Product Code: | RPPB0509 |
| Size: | 20µg |
| Species: | Rat |
| Target: | IL 4 |
| Synonyms: | BCGF, BCDF, B cell stimulating factor, BSF-1, Lymphocyte stimulatory factor 1, IL-4, MGC79402, Binetrakin, Pitrakinra. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation: | Lyophilized from a 0.2�m filtered concentrated solution in PBS pH 7.4 and 5% trehalose.�� |
| Solubility: | It is recommended to reconstitute the lyophilized Interleukin 4 in sterile 18M?-cm H2O not less than 100�g/ml, which can then be further diluted to other aqueous solutions. |
| Stability: | Lyophilized Interleukin-4 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IL4 should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles. |
| Purity: | Greater than 95.0% as determined by:(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE. |
| Amino Acid Sequence: | HGCNDSPLR EIINTLNQVT EKGTPCTEMF VPDVLTATRN TTENELICRA SRVLRKFYFP RDVPPCLKNK SGVLGELRKL CRGVSGLNSL RSCTVNESTL TTLKDFLESL KSILRGKYLQ SCTSMS |
| Biological Activity: | The ED50 as determined in a cell proliferation assay using rat splenocytes is less than 2ng/ml corresponding to a specific activity of 500,000IU/mg.� |
IL4 is a pleiotropic cytokine produced by activated T cells. IL4 is a ligand for interleukin 4 receptor. The interleukin 4 receptor also binds to IL13, which may contribute to many overlapping functions of this cytokine and IL13. STAT6, a signal transducer and activator of transcription, has been shown to play a central role in mediating the immune regulatory signal of this cytokine. This gene, IL3, IL5, IL13, and CSF2 form a cytokine gene cluster on chromosome 5q, with this gene particularly close to IL13. IL4, IL13 and IL5 are found to be regulated coordinately by several long-range regulatory elements in an over 120 kilobase range on the chromosome. Two alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported.
Interleukin-4 Rat Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 125 amino acids and having a molecular mass of 14kDa. The IL-4 is purified by proprietary chromatographic techniques.
| UniProt Protein Function: | IL4: Participates in at least several B-cell activation processes as well as of other cell types. It is a costimulator of DNA-synthesis. It induces the expression of class II MHC molecules on resting B-cells. It enhances both secretion and cell surface expression of IgE and IgG1. It also regulates the expression of the low affinity Fc receptor for IgE (CD23) on both lymphocytes and monocytes. Genetic variations in IL4 may be a cause of susceptibility to ischemic stroke (ISCHSTR); also known as cerebrovascular accident or cerebral infarction. A stroke is an acute neurologic event leading to death of neural tissue of the brain and resulting in loss of motor, sensory and/or cognitive function. Ischemic strokes, resulting from vascular occlusion, is considered to be a highly complex disease consisting of a group of heterogeneous disorders with multiple genetic and environmental risk factors. Belongs to the IL-4/IL-13 family. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Secreted, signal peptide; Cell cycle regulation; Secreted; Cytokine; Motility/polarity/chemotaxis Cellular Component: extracellular space; external side of plasma membrane Molecular Function:growth factor activity; cytokine activity; interleukin-4 receptor binding Biological Process: negative regulation of osteoclast differentiation; positive regulation of isotype switching to IgG isotypes; positive regulation of transcription, DNA-dependent; regulation of proton transport; microglial cell activation; female pregnancy; positive regulation of activated T cell proliferation; response to organic cyclic substance; positive regulation of interleukin-10 production; positive regulation of isotype switching to IgE isotypes; B cell costimulation; positive regulation of MHC class II biosynthetic process; positive regulation of cell proliferation; positive regulation of B cell proliferation; positive regulation of T cell proliferation; positive regulation of interleukin-13 production; negative regulation of macrophage activation; response to nutrient; response to drug; cholesterol metabolic process; regulation of immune response; negative regulation of chronic inflammatory response; B cell activation; negative regulation of nitric oxide biosynthetic process; negative regulation of acute inflammatory response; positive regulation of mast cell degranulation; defense response to protozoan; positive regulation of immunoglobulin production; T-helper 1 cell lineage commitment; positive regulation of peptidyl-tyrosine phosphorylation; positive regulation of chemokine biosynthetic process; response to ethanol; innate immune response in mucosa; positive regulation of tyrosine phosphorylation of Stat5 protein; positive regulation of T cell differentiation; retina development in camera-type eye; response to cytokine stimulus; positive regulation of B cell activation; regulation of inflammatory response; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription factor activity; T-helper 2 cell differentiation; positive regulation of protein amino acid phosphorylation; positive regulation of defense response to virus by host; negative regulation of transcription, DNA-dependent; negative regulation of T cell activation; regulation of T cell activation |
| NCBI Summary: | Th2-type cytokine; may be involved in inflammatory response in eosinophils [RGD, Feb 2006] |
| UniProt Code: | P20096 |
| NCBI GenInfo Identifier: | 124339 |
| NCBI Gene ID: | 287287 |
| NCBI Accession: | P20096.2 |
| UniProt Related Accession: | P20096 |
| Molecular Weight: | 16,247 Da |
| NCBI Full Name: | Interleukin-4 |
| NCBI Synonym Full Names: | interleukin 4 |
| NCBI Official Symbol: | Il4�� |
| NCBI Official Synonym Symbols: | Il4e12�� |
| NCBI Protein Information: | interleukin-4; IL-4; BSF-1; B-cell growth factor 1; B-cell stimulatory factor 1; lymphocyte stimulatory factor 1; B-cell IGG differentiation factor |
| UniProt Protein Name: | Interleukin-4 |
| UniProt Synonym Protein Names: | B-cell IGG differentiation factor; B-cell growth factor 1; B-cell stimulatory factor 1; BSF-1; Lymphocyte stimulatory factor 1 |
| Protein Family: | Interleukin |
| UniProt Gene Name: | Il4�� |
| UniProt Entry Name: | IL4_RAT |
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