Cytokines Recombinant Proteins

Rat IL 13 109 a.a. Recombinant Protein (RPPB0579)

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SKU:
RPPB0579
Product Type:
Recombinant Protein
Species:
Rat
Uniprot:
P42203
Research Area:
Cytokines

Description

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Product Name:Rat IL 13 109 a.a. Recombinant Protein
Product Code:RPPB0579
Size:10µg
Species:Rat
Target:IL 13 109 a.a.
Synonyms:NC300, ALRH, BHR1, P600, IL-13.
Source:Escherichia Coli
Physical Appearance:Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation:The protein (1mg/ml) was lyophilized in PBS, pH7.4.
Solubility:It is recommended to reconstitute the lyophilized Interleukin 13 in sterile 18M?-cm H2O not less than 100�g/ml, which can then be further diluted to other aqueous solutions.
Stability:Lyophilized Interleukin-13 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IL13 should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.
Purity:Greater than 95% as determined by SDS-PAGE.
Amino Acid Sequence:VRRSTSPPVA LRELIEELSN ITQDQKTSLC NSSIVWSVDI TAGGFCAALE SLTNISSCNA IHRTQRILNG LCNQKASDVA SSPPDTKIEV AQFISKLLNY SKQLFRYGH
Biological Activity:ED50 range = 40ng/ml, corresponding to a specific activity of > 25,000IU/mg as determined by the dose dependent proliferation of human TF-1 cells. Optimal concentration for individual application should be determined by a dose response assay.

IL13 is an immunoregulatory cytokine produced primarily by activated Th2 cells. IL-13 is involved in several stages of B-cell maturation and differentiation. It up-regulates CD23 and MHC class II expression, and promotes IgE isotype switching of B cells. This cytokine down-regulates macrophage activity, thereby inhibits the production of pro-inflammatory cytokines and chemokines. This cytokine is found to be critical to the pathogenesis of allergen-induced asthma but operates through mechanisms independent of IgE and eosinophils. This gene, IL3, IL5, IL4, and CSF2 form a cytokine gene cluster on chromosome 5q, with this gene particularly close to IL4.

Interleukin-13 Rat Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 109 amino acids and having a molecular mass of 11.9 kDa. The IL-13 is purified by proprietary chromatographic techniques.

UniProt Protein Function:IL13: Cytokine. Inhibits inflammatory cytokine production. Synergizes with IL2 in regulating interferon-gamma synthesis. May be critical in regulating inflammatory and immune responses. Defects in IL13 may be a cause of susceptibility to allergic rhinitis (ALRH). Allergic rhinitis is a common disease of complex inheritance and is characterized by mucosal inflammation caused by allergen exposure. Belongs to the IL-4/IL-13 family.
UniProt Protein Details:

Protein type:Motility/polarity/chemotaxis; Secreted; Secreted, signal peptide

Cellular Component: extracellular space; cytoplasm; extracellular region; external side of plasma membrane

Molecular Function:interleukin-13 receptor binding; cytokine activity

Biological Process: response to nicotine; positive regulation of smooth muscle cell proliferation; microglial cell activation; regulation of proton transport; positive regulation of mast cell degranulation; response to lipopolysaccharide; positive regulation of connective tissue growth factor production; positive regulation of tyrosine phosphorylation of Stat6 protein; positive regulation of immunoglobulin production; response to ethanol; positive regulation of protein secretion; response to mechanical stimulus; positive regulation of B cell proliferation; immune response; positive regulation of ion transport; positive regulation of release of sequestered calcium ion into cytosol; negative regulation of NAD(P)H oxidase activity; inflammatory response; positive regulation of macrophage activation

NCBI Summary:cytokine; involved in inflammatory and immune responses [RGD, Feb 2006]
UniProt Code:P42203
NCBI GenInfo Identifier:1170528
NCBI Gene ID:116553
NCBI Accession:P42203.1
UniProt Related Accession:P42203
Molecular Weight:14,093 Da
NCBI Full Name:Interleukin-13
NCBI Synonym Full Names:interleukin 13
NCBI Official Symbol:Il13��
NCBI Protein Information:interleukin-13; IL-13; T-cell activation protein P600
UniProt Protein Name:Interleukin-13
UniProt Synonym Protein Names:T-cell activation protein P600
Protein Family:Interleukin
UniProt Gene Name:Il13��
UniProt Entry Name:IL13_RAT
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