Cytokines Recombinant Proteins

Rat IL 10 Recombinant Protein (RPPB0556)

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RPPB0556

Description

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Product Name:Rat IL 10 Recombinant Protein
Product Code:RPPB0556
Size:10µg
Species:Rat
Target:IL 10
Synonyms:B-TCGF, CSIF, TGIF, IL-10, IL10A, MGC126450, MGC126451, Cytokine synthesis inhibitory factor.
Source:Escherichia Coli
Physical Appearance:Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation:The protein was lyophilized containing 20mM Tris-HCl pH-8.0 and 100mM NaCl.
Solubility:It is recommended to reconstitute the lyophilized IL-10 Rat Recombinant in sterile 18M?-cm H2O not less than 100 �g/ml, which can then be further diluted to other aqueous solutions.
Stability:Lyophilized IL-10 Rat although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Interleukin10 Rat recombinant should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.
Purity:Greater than 97.0% as determined by:(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE.
Amino Acid Sequence:SKGHSIRGDN NCTHFPVSQT HMLRELRAAF SQVKTFFQKK DQLDNILLTD SLLQDFKGYL GCQALSEMIK FYLVEVMPQA ENHGPEIKEH LNSLGEKLKT LWIQLRRCHR FLPCENKSKA VEQVKNDFNK LQDKGVYKAM NEFDIFINCI EAYVTLKMKN
Biological Activity:The ED50 as determined by the dose dependent inhibition of MC/9 proliferation is less than 10ng/ml concentration corresponding to a Specific Activity of 100,000IU/mg.

IL10 is a cytokine produced primarily by monocytes and to a lesser extent by lymphocytes. This cytokine has pleiotropic effects in immunoregulation and inflammation. It down-regulates the expression of Th1 cytokines, MHC class II Ags, and costimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. This cytokine can block NF-kappa B activity, and is involved in the regulation of the JAK-STAT signaling pathway. Knockout studies in mice suggested the function of this cytokine as an essential immunoregulator in the intestinal tract.

IL-10 Recombinant Rat produced in E.coli is a single, glycosylated polypeptide chain containing 160 amino acids and having a molecular mass of 18.6 kDa. The Interleukin-10 Mouse is purified by proprietary chromatographic techniques.

UniProt Protein Function:Inhibits the synthesis of a number of cytokines, including IFN-gamma, IL-2, IL-3, TNF and GM-CSF produced by activated macrophages and by helper T-cells.
NCBI Summary:factor involved in the inhibition of cytokine synthesis [RGD, Feb 2006]
UniProt Code:P29456
NCBI GenInfo Identifier:266338
NCBI Gene ID:25325
NCBI Accession:P29456.1
UniProt Secondary Accession:P29456,Q63263,
UniProt Related Accession:P29456
Molecular Weight:20,426 Da
NCBI Full Name:Interleukin-10
NCBI Synonym Full Names:interleukin 10
NCBI Official Symbol:Il10��
NCBI Official Synonym Symbols:IL10X��
NCBI Protein Information:interleukin-10; CSIF; IL-10; cytokine synthesis inhibitory factor
UniProt Protein Name:Interleukin-10
UniProt Synonym Protein Names:Cytokine synthesis inhibitory factor; CSIF
Protein Family:Interleukin
UniProt Gene Name:Il10��
UniProt Entry Name:IL10_RAT
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Additional Information

Product Type:
Recombinant Protein
Species:
Rat
Uniprot:
P29456
Research Area:
Cytokines
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