Growth Factors & Cytokines Recombinant Proteins
Rat IGF1 Recombinant Protein (RPPB0425)
- SKU:
- RPPB0425
- Product Type:
- Recombinant Protein
- Species:
- Rat
- Uniprot:
- P08025
- Research Area:
- Growth Factors & Cytokines
Description
| Product Name: | Rat IGF1 Recombinant Protein |
| Product Code: | RPPB0425 |
| Size: | 50µg |
| Species: | Rat |
| Target: | IGF1 |
| Synonyms: | Somatomedin C, IGF-I, IGFIA, IGF1. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation: | The protein was lyophilized with a 0.2�m filtered concentrated solution in 20mM PBS, pH 7.0. |
| Solubility: | It is recommended to reconstitute the lyophilized IGF1 in sterile 18M?-cm H2O not less than 100�g/ml, which can then be further diluted to other aqueous solutions. |
| Stability: | Lyophilized IGF-1 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IGFI should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles. |
| Purity: | Greater than 98.0% as determined by�SDS-PAGE. |
| Amino Acid Sequence: | GPETLCGAEL VDALQFVCGP RGFYFNKPTG YGSSIRRAPQ TGIVDECCFR SCDLRRLEMY CAPLKPTKSA |
| Biological Activity: | The ED50 as determined by a cell proliferation assay using FDC-P1 cells is less than 2.0ng/ml, corresponding to a specific activity of >500,000units/mg. |
The somatomedins, or IGFs, comprise a family of peptides that play important roles in mammalian growth and development. IGF1 mediates many of the growth-promoting effects of GH. Early studies showed that�GH did not directly stimulate the incorporation of sulfate into cartilage, but rather acted through a serum factor, termed 'sulfation factor,' which later became known as 'somatomedin' (Daughaday et al., 1972). Three main somatomedins have been characterized: somatomedin C (IGF1), somatomedin A (IGF2; MIM 147470), and somatomedin B (MIM 193190) (Rotwein, 1986; Rosenfeld, 2003).
IGF-1�Rat Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 70 amino acids and having a molecular mass of 7.7kDa. IGF-I is purified by proprietary chromatographic techniques.
| UniProt Protein Function: | The insulin-like growth factors, isolated from plasma, are structurally and functionally related to insulin but have a much higher growth-promoting activity. May be a physiological regulator of [1-14C]-2-deoxy-D-glucose (2DG) transport and glycogen synthesis in osteoblasts. Stimulates glucose transport in bone-derived osteoblastic (PyMS) cells and is effective at much lower concentrations than insulin, not only regarding glycogen and DNA synthesis but also with regard to enhancing glucose uptake. May play a role in synapse maturation. Ca2+-dependent exocytosis of IGF1 is required for sensory perception of smell in the olfactory bulb. Acts as a ligand for IGF1R. Binds to the alpha subunit of IGF1R, leading to the activation of the intrinsic tyrosine kinase activity which autophosphorylates tyrosine residues in the beta subunit thus initiatiating a cascade of down-stream signaling events leading to activation of the PI3K-AKT/PKB and the Ras-MAPK pathways. Binds to integrins ITGAV:ITGB3 and ITGA6:ITGB4. Its binding to integrins and subsequent ternary complex formation with integrins and IGFR1 are essential for IGF1 signaling. Induces the phosphorylation and activation of IGFR1, MAPK3/ERK1, MAPK1/ERK2 and AKT1. |
| NCBI Summary: | growth factor; plays a major role in mammalian growth [RGD, Feb 2006] |
| UniProt Code: | P08025 |
| NCBI GenInfo Identifier: | 124262 |
| NCBI Gene ID: | 24482 |
| NCBI Accession: | P08025.3 |
| UniProt Secondary Accession: | P08025,P08024, |
| UniProt Related Accession: | P08025 |
| Molecular Weight: | 17,831 Da |
| NCBI Full Name: | Insulin-like growth factor I |
| NCBI Synonym Full Names: | insulin-like growth factor 1 |
| NCBI Official Symbol: | Igf1�� |
| NCBI Protein Information: | insulin-like growth factor I; IGF-I; somatomedin |
| UniProt Protein Name: | Insulin-like growth factor I |
| UniProt Synonym Protein Names: | Somatomedin |
| Protein Family: | Insulin-like growth factor |
| UniProt Gene Name: | Igf1�� |
| UniProt Entry Name: | IGF1_RAT |
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