Growth Factors & Cytokines Recombinant Proteins

Rat IGF1 Recombinant Protein (RPPB0425)

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RPPB0425

Description

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Product Name:Rat IGF1 Recombinant Protein
Product Code:RPPB0425
Size:50µg
Species:Rat
Target:IGF1
Synonyms:Somatomedin C, IGF-I, IGFIA, IGF1.
Source:Escherichia Coli
Physical Appearance:Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation:The protein was lyophilized with a 0.2�m filtered concentrated solution in 20mM PBS, pH 7.0.
Solubility:It is recommended to reconstitute the lyophilized IGF1 in sterile 18M?-cm H2O not less than 100�g/ml, which can then be further diluted to other aqueous solutions.
Stability:Lyophilized IGF-1 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IGFI should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.
Purity:Greater than 98.0% as determined by�SDS-PAGE.
Amino Acid Sequence:GPETLCGAEL VDALQFVCGP RGFYFNKPTG YGSSIRRAPQ TGIVDECCFR SCDLRRLEMY CAPLKPTKSA
Biological Activity:The ED50 as determined by a cell proliferation assay using FDC-P1 cells is less than 2.0ng/ml, corresponding to a specific activity of >500,000units/mg.

The somatomedins, or IGFs, comprise a family of peptides that play important roles in mammalian growth and development. IGF1 mediates many of the growth-promoting effects of GH. Early studies showed that�GH did not directly stimulate the incorporation of sulfate into cartilage, but rather acted through a serum factor, termed 'sulfation factor,' which later became known as 'somatomedin' (Daughaday et al., 1972). Three main somatomedins have been characterized: somatomedin C (IGF1), somatomedin A (IGF2; MIM 147470), and somatomedin B (MIM 193190) (Rotwein, 1986; Rosenfeld, 2003).

IGF-1�Rat Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 70 amino acids and having a molecular mass of 7.7kDa. IGF-I is purified by proprietary chromatographic techniques.

UniProt Protein Function:The insulin-like growth factors, isolated from plasma, are structurally and functionally related to insulin but have a much higher growth-promoting activity. May be a physiological regulator of [1-14C]-2-deoxy-D-glucose (2DG) transport and glycogen synthesis in osteoblasts. Stimulates glucose transport in bone-derived osteoblastic (PyMS) cells and is effective at much lower concentrations than insulin, not only regarding glycogen and DNA synthesis but also with regard to enhancing glucose uptake. May play a role in synapse maturation. Ca2+-dependent exocytosis of IGF1 is required for sensory perception of smell in the olfactory bulb. Acts as a ligand for IGF1R. Binds to the alpha subunit of IGF1R, leading to the activation of the intrinsic tyrosine kinase activity which autophosphorylates tyrosine residues in the beta subunit thus initiatiating a cascade of down-stream signaling events leading to activation of the PI3K-AKT/PKB and the Ras-MAPK pathways. Binds to integrins ITGAV:ITGB3 and ITGA6:ITGB4. Its binding to integrins and subsequent ternary complex formation with integrins and IGFR1 are essential for IGF1 signaling. Induces the phosphorylation and activation of IGFR1, MAPK3/ERK1, MAPK1/ERK2 and AKT1.
NCBI Summary:growth factor; plays a major role in mammalian growth [RGD, Feb 2006]
UniProt Code:P08025
NCBI GenInfo Identifier:124262
NCBI Gene ID:24482
NCBI Accession:P08025.3
UniProt Secondary Accession:P08025,P08024,
UniProt Related Accession:P08025
Molecular Weight:17,831 Da
NCBI Full Name:Insulin-like growth factor I
NCBI Synonym Full Names:insulin-like growth factor 1
NCBI Official Symbol:Igf1��
NCBI Protein Information:insulin-like growth factor I; IGF-I; somatomedin
UniProt Protein Name:Insulin-like growth factor I
UniProt Synonym Protein Names:Somatomedin
Protein Family:Insulin-like growth factor
UniProt Gene Name:Igf1��
UniProt Entry Name:IGF1_RAT
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Additional Information

Product Type:
Recombinant Protein
Species:
Rat
Uniprot:
P08025
Research Area:
Growth Factors & Cytokines
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