Growth Factors & Cytokines Recombinant Proteins

Rat HB-EGF Recombinant Protein (RPPB0271)

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SKU:
RPPB0271
Product Type:
Recombinant Protein
Species:
Rat
Uniprot:
Q06175
Research Area:
Growth Factors & Cytokines

Description

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Product Name:Rat HB-EGF Recombinant Protein
Product Code:RPPB0271
Size:50µg
Species:Rat
Target:HB-EGF
Synonyms:Proheparin-binding EGF-like growth factor, Heparin-binding EGF-like growth factor, HB-EGF, HBEGF, Dtr, Hegfl, GFHB.
Source:Escherichia Coli
Physical Appearance:Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation:The protein was filtered (0.2�m) and lyophilized from a concentrated solution containing PBS, 300mM NaCl, pH 7.4 and 5% trehalose.
Solubility:It is recommended to reconstitute the lyophilized�Rat HB-EGF in sterile 18M-cm H2O not less than 100 �g/ml, which can then be further diluted to other aqueous solutions.
Stability:Lyophilized�Rat HB-EGF Recombinant although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution HB-EGF should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.
Purity:Greater than 95.0% as determined by:(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE.
Amino Acid Sequence:DLEGTDLDLF KVAFSSKPQA LATPGKEKNG KKKRKGKGLG KKRDPCLKKY KDYCIHGECR YLKELRIPSC HCLPGYHGQR CHGLTL
Biological Activity:The ED50 as determined by a cell proliferation assay using balb/c 3T3 cells is < 1.0 ng/ml, corresponding to a specific activity of > 1.0�106 units/mg.

HB-EGF is an EGF related growth factor which signals via the EGF receptor, and stimulates the proliferation of SMC (smooth muscle cells), fibroblasts, epithelial cells and keratinocytes. HB-EGF is expressed in various cell types and tissues, including vascular endothelial cells and SMC, macrophages, skeletal muscle, keratinocytes and particular tumor cells. HB-EGF�s ability to explicitly bind heparin and heparin sulfate proteoglycans is dissimilar from other EGF-like molecules, and might be related to the enhanced mitogenic activity, relative to EGF, that HB-EGF exerts on smooth muscle cells.

HB-EGF Rat Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 86 amino acids and having a molecular mass of 9.7kDa.The HB-EGF is purified by proprietary chromatographic techniques.

UniProt Protein Function:Function: Growth factor that mediates its effects via EGFR, ERBB2 and ERBB4. Required for normal cardiac valve formation and normal heart function. Promotes smooth muscle cell proliferation. May be involved in macrophage-mediated cellular proliferation. It is mitogenic for fibroblasts, but not endothelial cells. It is able to bind EGF receptor/EGFR with higher affinity than EGF itself and is a far more potent mitogen for smooth muscle cells than EGF. Also acts as a diphtheria toxin receptor
NCBI Summary:activates epidermal growth factor receptor mediated signaling; contributes to neuronal survival; may play a role in neurogenesis during ventral midbrain development [RGD, Feb 2006]
UniProt Code:Q06175
NCBI GenInfo Identifier:6978785
NCBI Gene ID:25433
NCBI Accession:NP_037077.1
UniProt Related Accession:Q06175
Molecular Weight:22,843 Da
NCBI Full Name:proheparin-binding EGF-like growth factor
NCBI Synonym Full Names:heparin-binding EGF-like growth factor
NCBI Official Symbol:Hbegf��
NCBI Official Synonym Symbols:Dtr; GFHB; Hegfl; Hb-egf��
NCBI Protein Information:proheparin-binding EGF-like growth factor; heparin-binding epidermal -growth factor; heparin-binding epidermal -growth - like growth factor; heparin binding epidermal growth factor-like growth factor; Diphtheria toxin receptor (heparin binding epidermal growth factor - like growth factor)
UniProt Protein Name:Proheparin-binding EGF-like growth factor
Protein Family:Proheparin-binding EGF-like growth factor
UniProt Gene Name:Hbegf��
UniProt Entry Name:HBEGF_RAT
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