Rat Growth Factor Array (14 targets) (SARB0103)

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SKU:
SARB0103

Description

system_update_altDatasheet
Product Sku:SARB0103
Size:2, 4, or 8
Species Detected:Rat
Number of Targets Detected:14
Gene Symbols:BDNF, CSF2, EGF, FGF2, FLT3LG, GDNF, HGF, IGF1, IGFBP5, INHBA|NGF, PDGFA, PDGFB, VEGFA
Compatible Sample Types:Cell Culture Supernatants, Cell Lysates, Plasma, Serum, Tissue Lysates
Design Principle:Sandwich-based
Method of Detection:Chemiluminescence
Pathway:
Quantitative/Semi-Quantitative:Semi-Quantitative
Solid Support:Membrane
Suggested Application(s):Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process; Detecting Nephrotoxicity
Storage/Stability:For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period.
Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
Hover over target for more information
Activin A
  • Gene Symbol: Inhba
  • Gene ID: 29200
  • Accession #: P18331
BDNF
  • Gene Symbol: Bdnf
  • Gene ID: 24225
  • Accession #: P23363
beta-NGF
  • Gene Symbol: Ngf, Ngfb
  • Gene ID:
  • Accession #: P25427
bFGF
  • Gene Symbol: Fgf2 Fgf-2
  • Gene ID: 54250
  • Accession #: P13109
EGF
  • Gene Symbol: Egf
  • Gene ID: 25313
  • Accession #: P07522
Flt-3 Ligand
  • Gene Symbol: N/A
  • Gene ID: N/A
  • Accession #: N/A
GDNF
  • Gene Symbol: Gdnf
  • Gene ID: 25453
  • Accession #: Q07731
GM-CSF
  • Gene Symbol: Csf2 Csfgm
  • Gene ID: 116630
  • Accession #: P48750
HGF
  • Gene Symbol: Hgf
  • Gene ID: 24446
  • Accession #: P17945
IGF-1
  • Gene Symbol: Igf1 Igf-1
  • Gene ID: 24482
  • Accession #: P08025
PDGF-AA
  • Gene Symbol: Pdgfa rCG_42712
  • Gene ID: 25266
  • Accession #: Q6P7C3
PDGF-BB
  • Gene Symbol: N/A
  • Gene ID: N/A
  • Accession #: N/A
IGFBP-5
  • Gene Symbol: Igfbp5 Igfbp-5
  • Gene ID: 25285
  • Accession #: P24594
VEGF-A
  • Gene Symbol: Vegfa Vegf
  • Gene ID: 83785
  • Accession #: P16612
  • Easy to use
  • No specialized equipment needed
  • Compatible with nearly any liquid sample
  • Proven technology
  • Highly sensitive (pg/ml)
  • Sandwich ELISA specificity
  • Higher density than ELISA, Western blot or bead-based multiplex
  • Rat Growth Factor Array 1
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated HRP
  • Detection Buffer C
  • Detection Buffer D
  • Lysis Buffer
  • 8-Well Incubation Tray
  • Plastic Sheets
  • Array Templates

Other Materials Required

  • Pipettors, pipet tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Tissue Paper, blotting paper or chromatography paper
  • Adhesive tape or Saran Wrap
  • Distilled or de-ionized water
  • A chemiluminescent blot documentation system , X-ray Film and a suitable film processor, or another chemiluminescent detection system.
1.Block membranes
2.Incubate with Sample
3.Incubate with Biotinylated Detection Antibody Cocktail
4.Incubate with HRP-Conjugated Streptavidin
5.Incubate with Detection Buffers
6.Image with chemiluminescent imaging system
7.Perform densitometry and analysis
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Additional Information

Product Type:
Protein Array
Analytes:
Activin A
Analytes:
BDNF
Analytes:
beta-NGF
Analytes:
bFGF
Analytes:
EGF
Analytes:
Flt-3 Ligand
Analytes:
GDNF
Analytes:
GM-CSF
Analytes:
HGF
Analytes:
IGF-1
Analytes:
PDGF-AA
Analytes:
PDGF-BB
Analytes:
IGFBP-5
Analytes:
VEGF-A
Reactivity:
Rat
Applications:
Multiplex Array
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