Enzymes Recombinant Proteins

Rat GPT Recombinant Protein (RPPB1738)

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SKU:
RPPB1738
Product Type:
Recombinant Protein
Species:
Rat
Uniprot:
P25409
Research Area:
Enzymes

Description

system_update_altDatasheetsystem_update_altMSDS
Product Name:Rat GPT Recombinant Protein
Product Code:RPPB1738
Size:20µg
Species:Rat
Target:GPT
Synonyms:Alanine aminotransferase 1 (EC:2.6.1.2), ALT1, Glutamate pyruvate transaminase 1, GPT 1, Glutamic--alaninetransaminase 1, Glutamic--pyruvic transaminase 1, Gpt, Aat1, Gpt1.
Source:Escherichia Coli
Physical Appearance:Sterile Filtered colorless solution.
Formulation:GPT protein solution (1mg/ml) containing PhosphateBuffered Saline pH 7.4 and 10% glycerol.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Purity:Greaterthan 90.0% as determined by SDS-PAGE.
Amino Acid Sequence:MGSSHHHHHHSSGLVPRGSH MGSMASRVND QSQASRNGLKGKVLTLDTMN PCVRRVEYAV RGPIVQRALE LEQELRQGVK KPFTEVIRAN IGDAQAMGQR PITFFRQVLALCVYPNLLSS PDFPEDAKRR AERILQACGG HSLGAYSISS GIQPIREDVA QYIERRDGGI PADPNNIFLS TGASDAIVTMLKLLVSGEGR ARTGVLIPIP QYPLYSAALA ELDAVQVDYY LDEERAWALD IAELRRALCQ ARDRCCPRVLCVINPGNPTG QVQTRECIEA VIRFAFKEGL FLMADEVYQD NVYAEGSQFH SFKKVLMEMG PPYSTQQELASFHSVSKGYM GECGFRGGYV EVVNMDAEVQ KQMGKLMSVR LCPPVPGQAL MDMVVSPPTP SEPSFKQFQAERQEVLAELA AKAKLTEQVF NEAPGIRCNP VQGAMYSFPQ VQLPLKAVQR AQELGLAPDM FFCLCLLEETGICVVPGSGF GQQEGTYHFR MTILPPMEKL RLLLEKLSHF HAKFTHEYS
Biological Activity:Specific activity is> 60units/mg, and is defined as the amount of enzyme that cleaves 1umole ofL-Alanine to L-Glutamate per minute at pH 7.5 at 37C.

GPT catalyzes the reversible transamination between alanine and 2-oxoglutarate to create pyruvate and glutamate. GPT has a crucial part in the intermediary metabolism of glucose and amino acids. GPT is broadly used as an indicator of liver reliability or hepatocellular destruction in clinical tests.

GPT Rat Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 519 amino acids (1-496 a.a) and having a molecular mass of 57.5kDa.�GPT is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

UniProt Protein Function:GPT: Catalyzes the reversible transamination between alanine and 2-oxoglutarate to form pyruvate and glutamate. Participates in cellular nitrogen metabolism and also in liver gluconeogenesis starting with precursors transported from skeletal muscles. Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family. Alanine aminotransferase subfamily.
UniProt Protein Details:

Protein type:Amino Acid Metabolism - alanine, aspartate and glutamate; EC 2.6.1.2; Transferase

Chromosomal Location of Human Ortholog: 7q34

Cellular Component: cytoplasm; extracellular space

Molecular Function:L-alanine:2-oxoglutarate aminotransferase activity; pyridoxal phosphate binding

Biological Process: biosynthetic process; L-alanine catabolic process

NCBI Summary:human homolog is a cytosolic alanine aminotransferase [RGD, Feb 2006]
UniProt Code:P25409
NCBI GenInfo Identifier:13591961
NCBI Gene ID:81670
NCBI Accession:NP_112301.1
UniProt Secondary Accession:P25409,Q4V7F7,
UniProt Related Accession:P25409
Molecular Weight:55,110 Da
NCBI Full Name:alanine aminotransferase 1
NCBI Synonym Full Names:glutamic--pyruvic transaminase
NCBI Official Symbol:Gpt��
NCBI Official Synonym Symbols:ALAT; Gpt1��
NCBI Protein Information:alanine aminotransferase 1
UniProt Protein Name:Alanine aminotransferase 1
UniProt Synonym Protein Names:Glutamate pyruvate transaminase 1; GPT 1; Glutamic--alanine transaminase 1; Glutamic--pyruvic transaminase 1
Protein Family:NAD--protein ADP-ribosyltransferase
UniProt Gene Name:Gpt��
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system_update_altDatasheetRat GPT1/GPT Recombinant Protein Alanine aminotransferase (ALT), also known as glutamate pyruvate transaminase (GPT), is a pyridoxal enzyme which belongs to the class-I