Rat Fractalkine PharmaGenie ELISA Kit (SBRS1636)

(No reviews yet) Write a Review
SKU:
SBRS1636
Product Type:
ELISA Kit
Reactivity:
Rat
Applications:
ELISA
ELISA Type:
Sandwich

Description

system_update_altDatasheet

Rat Fractalkine PharmaGenie ELISA Kit (SBRS1636)

Product SKU:SBRS1636
Size:96T
Application:This ELISA kit shows no cross-reactivity with any of the cytokines tested: rat CINC-2, CINC-3, CNTF, IL-1 alpha, IL-1 beta, IL-4, IL-6, IL-10, GM-CSF, IFN-gamma, Leptin, Lix, MCP-1, MIP-3 alpha, beta-NGF, TIMP-1, TNF-alpha, VEGF.
Uniprot:Q6IRF7
Gene Names:CX3CL1 / RCG_39059
Synonyms:Chemokine (C-X3-C motif) ligand 1 (Cx3cl1 protein) (Fractalkine)
Target Species:Rat
Compatible Sample Types:Cell and Tissue Lysates
Design Principle:Sandwich-based
Method of Detection:Colorimetric
Quantitative/Semi-Quantitative:Quantitative
Range:5 pg/ml - 2000 pg/ml
Sensitivity:5 pg/ml
Recommended Dilution:3 fold
Storage/Stability:The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
  1. Pre-Coated 96-well Strip Microplate
  2. Wash Buffer
  3. Stop Solution
  4. Assay Diluent(s)
  5. Lyophilized Standard
  6. Biotinylated Detection Antibody
  7. Streptavidin-Conjugated HRP
  8. TMB One-Step Substrate

Other materials and equipment required:

The Assay Genie Rat Fractalkine PharmaGenie ELISA Kit (SBRS1636) will require other equipment and materials to carry out the assay. Please see list below for further details.

  • Distilled or deionized water
  • Precision pipettes to deliver 2 ul to 1 ul volumes
  • Adjustable 1-25 ul pipettes for reagent preparation
  • 100 ul and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 ul of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4°C.
  4. Add 100 ul of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 ul of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 ul of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 ul of Stop Solution to each well.
  11. Read at 450 nm immediately.
View AllClose

0 Reviews

View AllClose

Related Products

Rat GIP PharmaGenie ELISA Kit (SBRS0133)

Rat GIP PharmaGenie ELISA Kit (SBRS0133)

Rat GIP PharmaGenie ELISA - Kit InformationGastric inhibitory polypeptide (GIP), also known as the glucose-dependent insulinotropic peptide belongs to the secretin family. GIP is derived from a...
Rat Obestatin PharmaGenie ELISA Kit (SBRS0148)

Rat Obestatin PharmaGenie ELISA Kit (SBRS0148)

Rat Obestatin PharmaGenie ELISA - Kit InformationObestatin is a 24-aa peptide hormone that is produced in the stomach and small intestine of humans and several other mammals. It is encoded by the...