Growth Factors & Cytokines Recombinant Proteins

Rat EGF Protein Recombinant Protein (RPPB0180)

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SKU:
RPPB0180
Product Type:
Recombinant Protein
Species:
Rat
Uniprot:
P07522
Research Area:
Growth Factors & Cytokines

Description

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Product Name:Rat EGF Protein Recombinant Protein
Product Code:RPPB0180
Size:5µg
Species:Rat
Target:EGF Protein
Synonyms:Urogastrone, URG, EGF.
Source:Adult Male Rat Submandibular Glands
Physical Appearance:Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation:The protein was lyophilized from a concentrated (1mg/ml) solution containing 0.01M sodium acetate buffer.
Solubility:It is recommended to reconstitute the lyophilized Epidermal Growth Factor in sterile 18M?-cm H2O not less than 100�g/ml, which can then be further diluted to other aqueous solutions.
Stability:Lyophilized Epidermal Growth Factor Recombinant although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution EGF should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.
Purity:Greater than 99.0% as determined by(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE.

Epidermal growth factor has a profound effect on the differentiation of specific cells in vivo and is a potent mitogenic factor for a variety of cultured cells of both ectodermal and mesodermal origin. The EGF precursor is believed to exist as a membrane-bound molecule which is proteolytically cleaved to generate the 53-amino acid peptide hormone that stimulates cells to divide.EGF stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture.

Epidermal Growth Factor Rat purified from submandibular gland is a single, glycosylated, polypeptide chain having a molecular mass of 6.15 kDa.The EGF is purified by proprietary chromatographic techniques.

UniProt Protein Function:EGF: EGF stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture. Magnesiotropic hormone that stimulates magnesium reabsorption in the renal distal convoluted tubule via engagement of EGFR and activation of the magnesium channel TRPM6. Defects in EGF are the cause of hypomagnesemia type 4 (HOMG4); also known as renal hypomagnesemia normocalciuric. HOMG4 is a disorder characterized by massive renal hypomagnesemia and normal levels of serum calcium and calcium excretion. Clinical features include seizures, mild-to mederate psychomotor retardation, and brisk tendon reflexes. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Ligand, receptor tyrosine kinase; Membrane protein, integral; Hormone

Cellular Component: extracellular space; lysosomal membrane; plasma membrane; integral to membrane; intracellular

Molecular Function:growth factor activity; calcium ion binding; epidermal growth factor receptor binding

Biological Process: epidermal growth factor receptor signaling pathway; peptidyl-tyrosine phosphorylation; positive regulation of mitosis; activation of MAPKK activity; positive regulation of transcription, DNA-dependent; regulation of protein transport; positive regulation of MAP kinase activity; regulation of protein secretion; positive regulation of epidermal growth factor receptor activity; branching morphogenesis of a tube; positive regulation of cell proliferation; negative regulation of secretion; angiogenesis; regulation of peptidyl-tyrosine phosphorylation; positive regulation of phosphorylation; positive regulation of DNA binding; STAT protein nuclear translocation; positive regulation of granule cell precursor proliferation

NCBI Summary:binds the epidermal growth factor receptor; may play a role in MAP kinase mediated signaling pathways [RGD, Feb 2006]
UniProt Code:P07522
NCBI GenInfo Identifier:1352360
NCBI Gene ID:25313
NCBI Accession:P07522.2
UniProt Secondary Accession:P07522,Q63183,
UniProt Related Accession:P07522
Molecular Weight:124,126 Da
NCBI Full Name:Pro-epidermal growth factor
NCBI Synonym Full Names:epidermal growth factor
NCBI Official Symbol:Egf��
NCBI Protein Information:pro-epidermal growth factor
UniProt Protein Name:Pro-epidermal growth factor
Protein Family:EGF-like domain containing protein
UniProt Gene Name:Egf��
UniProt Entry Name:EGF_RAT
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