Rat DKK-1 PharmaGenie ELISA Kit (SBRS1626)

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SKU:
SBRS1626
Product Type:
ELISA Kit
Reactivity:
Rat
Applications:
ELISA
ELISA Type:
Sandwich

Description

system_update_altDatasheet

Rat DKK-1 PharmaGenie ELISA Kit (SBRS1626)

Product SKU:SBRS1626
Size:96T
Application:This ELISA antibody pair recognizes Rat DKK-1.
Uniprot:D3Z9J1
Gene ID:293897 / 108348170
Gene Names:DKK1 / DKK1_PREDICTED / LOC100912050 / NEWGENE_1307313 / RCG_48380
Synonyms:Dickkopf WNT-signaling pathway inhibitor 1 (Dickkopf homolog 1 (Xenopus laevis)) (Dickkopf-like protein 1)
Target Species:Rat
Design Principle:Sandwich-based
Method of Detection:Colorimetric
Quantitative/Semi-Quantitative:Quantitative
Range:33 pg/ml - 8000 pg/ml
Sensitivity:33 pg/ml
Recommended Dilution:2 fold
Storage/Stability:May be stored for up to 6 months at 2 to 8°C from the date of shipment. Standard (recombinant protein) should be stored at -20°C or -80°C (recommended at -80°C) after reconstitution. Opened Microplate Wells or reagents may be store for up to 1 month at 2 to 8°C. Return unused wells to the pouch containing desiccant pack, reseal along entire edge. Note: the kit can be used within one year if the whole kit is stored at -20°C.
  1. Pre-Coated 96-well Strip Microplate
  2. Wash Buffer
  3. Stop Solution
  4. Assay Diluent(s)
  5. Lyophilized Standard
  6. Biotinylated Detection Antibody
  7. Streptavidin-Conjugated HRP
  8. TMB One-Step Substrate

Other materials and equipment required:

The Assay Genie Rat DKK-1 PharmaGenie ELISA Kit (SBRS1626) will require other equipment and materials to carry out the assay. Please see list below for further details.

  • Distilled or deionized water
  • Precision pipettes to deliver 2 ul to 1 ul volumes
  • Adjustable 1-25 ul pipettes for reagent preparation
  • 100 ul and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 ul of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4°C.
  4. Add 100 ul of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 ul of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 ul of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 ul of Stop Solution to each well.
  11. Read at 450 nm immediately.
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