Rat DHEA-S (Dehydroepiandrosterone Sulfate) ELISA Kit
- SKU:
- AEES00377
- Product Type:
- ELISA Kit
- ELISA Type:
- Competitive
- Size:
- 96 Assays
- Reactivity:
- Rat
- Sensitivity:
- 18.75 pg/mL
- Range:
- 31.25-2000 pg/mL
- Sample Type:
- Serum, plasma and other biological fluids
Description
| Product Name: | Rat DHEA-S (Dehydroepiandrosterone Sulfate) ELISA Kit |
| Product Code: | AEES00377 |
| Assay Type: | Competitive |
| Format: | 96T |
| Assay Time: | 2.0h |
| Reactivity: | Rat |
| Detection Range: | 31.25-2000 pg/mL |
| Sensitivity: | 18.75 pg/mL |
| Sample Type & Sample Volume: | Serum, plasma and other biological fluids, 50μL |
| Specificity: | This kit recognizes DHEA-S in samples. No significant cross-reactivity or interference between DHEA-S and analogues was observed. |
| Reproducibility: | Both intra-CV and inter-CV are < 10%. |
| Application: | This ELISA kit applies to the in vitro quantitative determination of DHEA-S concentrations in serum, plasma and other biological fluids. |
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with DHEA-S. During the reaction, DHEA-S in the sample or standard competes with a fixed amount of DHEA-S on the solid phase supporter for sites on the Biotinylated Detection Ab specific to DHEA-S. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of DHEA-S in tested samples can be calculated by comparing the OD of the samples to the standard curve.
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level DHEA-S were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level DHEA-S were tested on 3 different plates, 20 replicates in each plate.
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (pg/mL) | 95.90 | 174.40 | 936.30 | 89.90 | 166.70 | 1012.30 |
| Standard deviation | 5.10 | 7.50 | 36.50 | 5.20 | 7.30 | 48.60 |
| CV (%) | 5.32 | 4.30 | 3.90 | 5.78 | 4.38 | 4.80 |
Recovery
The recovery of DHEA-S spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 89-103 | 94 |
| EDTA plasma(n=8) | 86-99 | 91 |
| Cell culture media(n=8) | 86-100 | 91 |
| Kit Components: | An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.
|
| 1. | Add 50μL standard or sample to the wells, immediately add 50μL Biotinylated Detection Ab working solution to each well. Incubate for 45 min at 37°C |
| 2. | Aspirate and wash the plate for 3 times |
| 3. | Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times |
| 4. | Add 90μL Substrate Reagent. Incubate for 15 min at 37°C |
| 5. | Add 50μL Stop Solution |
| 6. | Read the plate at 450nm immediately. Calculation of the results |
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