Rat Cytokine Array (34 targets) (SARB0102)

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SKU:
SARB0102

Description

system_update_altDatasheet
Product Sku:SARB0102
Size:2, 4, or 8
Species Detected:Rat
Number of Targets Detected:34
Gene Symbols:AGER, AGRN, CCL2, CCL20, CD86, CNTF, CSF2, CX3CL1, CXCL1, CXCL2|CXCL3, CXCL5, FASLG, ICAM1, IFNG, IL10, IL13, IL1A, IL1B, IL1RL2, IL2, IL4, IL6, INHBA, LEP, MMP8, NGF, PDGFA, PPBP, PRLR, SELL, TIMP1, TNF, VEGFA
Compatible Sample Types:Cell Culture Supernatants, Cell Lysates, Plasma, Serum, Tissue Lysates
Design Principle:Sandwich-based
Method of Detection:Chemiluminescence
Pathway:
Quantitative/Semi-Quantitative:Semi-Quantitative
Solid Support:Membrane
Suggested Application(s):Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Storage/Stability:For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period.
Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
Hover over target for more information
Activin A
  • Gene Symbol: INHBA
  • Gene ID: N/A
  • Accession #: P18331
Agrin
  • Gene Symbol: AGRN
  • Gene ID: N/A
  • Accession #: P25304
CD86 (B7-2)
  • Gene Symbol: CD86
  • Gene ID: N/A
  • Accession #: O35531
beta-NGF
  • Gene Symbol: NGF
  • Gene ID: N/A
  • Accession #: P25427
CINC-1 (CXCL1)
  • Gene Symbol: CXCL1
  • Gene ID: N/A
  • Accession #: P14095
CINC-2
  • Gene Symbol: CXCL3
  • Gene ID: N/A
  • Accession #: Q10746
CINC-3
  • Gene Symbol: Cxcl2
  • Gene ID: N/A
  • Accession #: P30348
CNTF
  • Gene Symbol: CNTF
  • Gene ID: N/A
  • Accession #: P20294
Fas Ligand (TNFSF6)
  • Gene Symbol: FASLG
  • Gene ID: N/A
  • Accession #: P36940
Fractalkine (CX3CL1)
  • Gene Symbol: CX3CL1
  • Gene ID: N/A
  • Accession #: Q6IRF7
GM-CSF
  • Gene Symbol: CSF2
  • Gene ID: N/A
  • Accession #: P48750
ICAM-1 (CD54)
  • Gene Symbol: ICAM1
  • Gene ID: N/A
  • Accession #: Q00238
IFN-gamma
  • Gene Symbol: IFNG
  • Gene ID: N/A
  • Accession #: P01581
IL-1 alpha (IL-1 F1)
  • Gene Symbol: IL1A
  • Gene ID: N/A
  • Accession #: P16598
IL-1 beta (IL-1 F2)
  • Gene Symbol: IL1B
  • Gene ID: N/A
  • Accession #: Q63264
IL-1 R6 (IL-1 Rrp2)
  • Gene Symbol: IL1RL2
  • Gene ID: N/A
  • Accession #: Q62929
IL-2
  • Gene Symbol: IL2
  • Gene ID: N/A
  • Accession #: P17108
IL-4
  • Gene Symbol: IL4
  • Gene ID: N/A
  • Accession #: P20096
IL-6
  • Gene Symbol: IL6
  • Gene ID: N/A
  • Accession #: P20607
IL-10
  • Gene Symbol: IL10
  • Gene ID: N/A
  • Accession #: P29456
IL-13
  • Gene Symbol: IL13
  • Gene ID: N/A
  • Accession #: P42203
Leptin
  • Gene Symbol: LEP
  • Gene ID: N/A
  • Accession #: P50596
LIX
  • Gene Symbol: Cxcl5
  • Gene ID: N/A
  • Accession #: P97885
L-Selectin (CD62L)
  • Gene Symbol: SELL
  • Gene ID: N/A
  • Accession #: P30836
MCP-1 (CCL2)
  • Gene Symbol: CCL2
  • Gene ID: N/A
  • Accession #: P14844
MIP-3 alpha (CCL20)
  • Gene Symbol: CCL20
  • Gene ID: N/A
  • Accession #: P97884
MMP-8
  • Gene Symbol: MMP8
  • Gene ID: N/A
  • Accession #: O88766
PDGF-AA
  • Gene Symbol: PDGFA
  • Gene ID: N/A
  • Accession #: Q6P7C3
Prolactin R
  • Gene Symbol: Prlr
  • Gene ID: N/A
  • Accession #: P05710
RAGE
  • Gene Symbol: MOK
  • Gene ID: N/A
  • Accession #: Q63495
TCK-1 (CXCL7)
  • Gene Symbol: Ppbp
  • Gene ID: N/A
  • Accession #: Q99ME0
TIMP-1
  • Gene Symbol: TIMP1
  • Gene ID: N/A
  • Accession #: P30120
TNF alpha
  • Gene Symbol: TNF
  • Gene ID: N/A
  • Accession #: P16599
VEGF-A
  • Gene Symbol: VEGFA
  • Gene ID: N/A
  • Accession #: P16612
  • Easy to use
  • No specialized equipment needed
  • Compatible with nearly any liquid sample
  • Proven technology
  • Highly sensitive (pg/ml)
  • Sandwich ELISA specificity
  • Higher density than ELISA, Western blot or bead-based multiplex
  • Rat Cytokine Antibody Array C2 Membranes
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated HRP
  • Detection Buffer C
  • Detection Buffer D
  • Lysis Buffer
  • 8-Well Incubation Tray
  • Plastic Sheets
  • Array Templates

Other Materials Required

  • Pipettors, pipet tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Tissue Paper, blotting paper or chromatography paper
  • Adhesive tape or Saran Wrap
  • Distilled or de-ionized water
  • A chemiluminescent blot documentation system , X-ray Film and a suitable film processor, or another chemiluminescent detection system.
1.Block membranes
2.Incubate with Sample
3.Incubate with Biotinylated Detection Antibody Cocktail
4.Incubate with HRP-Conjugated Streptavidin
5.Incubate with Detection Buffers
6.Image with chemiluminescent imaging system
7.Perform densitometry and analysis
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Additional Information

Product Type:
Protein Array
Analytes:
Activin A
Analytes:
Agrin
Analytes:
CD86 (B7-2)
Analytes:
beta-NGF
Analytes:
CINC-1 (CXCL1)
Analytes:
CINC-2
Analytes:
CINC-3
Analytes:
CNTF
Analytes:
Fas Ligand (TNFSF6)
Analytes:
Fractalkine (CX3CL1)
Analytes:
GM-CSF
Analytes:
ICAM-1 (CD54)
Analytes:
IFN-gamma
Analytes:
IL-1 alpha (IL-1 F1)
Analytes:
IL-1 beta (IL-1 F2)
Analytes:
IL-1 R6 (IL-1 Rrp2)
Analytes:
IL-2
Analytes:
IL-4
Analytes:
IL-6
Analytes:
IL-10
Analytes:
IL-13
Analytes:
Leptin
Analytes:
LIX
Analytes:
L-Selectin (CD62L)
Analytes:
MCP-1 (CCL2)
Analytes:
MIP-3 alpha (CCL20)
Analytes:
MMP-8
Analytes:
PDGF-AA
Analytes:
Prolactin R
Analytes:
RAGE
Analytes:
TCK-1 (CXCL7)
Analytes:
TIMP-1
Analytes:
TNF alpha
Analytes:
VEGF-A
Reactivity:
Rat
Applications:
Multiplex Array
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