Enzymes Recombinant Proteins
Rat Cyclophilin F Bioactive Recombinant Protein (RPPB1544)
- SKU:
- RPPB1544
- Product Type:
- Recombinant Protein
- Species:
- Rat
- Uniprot:
- P29117
- Research Area:
- Enzymes
Description
| Product Name: | Rat Cyclophilin F Bioactive Recombinant Protein |
| Product Code: | RPPB1544 |
| Size: | 10µg |
| Species: | Rat |
| Target: | Cyclophilin F Bioactive |
| Synonyms: | Peptidyl-prolyl cis-trans isomerase F, mitochondrial, PPIaseF, Cyclophilin D, CyP-D, CypD, Cyclophilin F, Rotamase F. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile filtered colorless solution. |
| Formulation: | CyclophilinF protein solution (1mg/ml) containing Phosphate Buffered Saline (pH 7.4), 10%glycerol and 1mM DTT. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks.�Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greaterthan 90.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MGSCSDGGAR GANSSSQNPL VYLDVGADGQ PLGRVVLELK ADVVPKTAENFRALCTGEKG FGYKGSTFHR VIPAFMCQAG DFTNHNGTGG KSIYGSRFPD ENFTLKHVGP GVLSMANAGPNTNGSQFFIC TIKTDWLDGK HVVFGHVKEG MDVVKKIESFGSKSGKTSKK IVITDCGQLS |
| Biological Activity: | Specificactivity is > 1,300 nmol/min/mg, and is defined as the amount of enzyme thatcleaves 1nmol of suc-AAFP-PNA per minute at 37C in Tris�HCl pH 8.0 using chymotrypsin. |
| UniProt Code: | P29117 |
PPIF is a part of the peptidyl-prolyl cis-trans isomerase (PPIase) family. PPIF accelerates the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. PPIF is key component of the mitochondrial permeability transition pore in the inner mitochondrial membrane. Activation of this pore is thought to be involved in the induction of apoptotic and necrotic cell death.
Cyclophilin F Rat Recombinant produced in E.Coli is asingle, non-glycosylated polypeptide chain containing 200 amino acids (30-206 a.a)and having a molecular mass of 21.2Da.Cyclophilin F is fused to a23 amino acid His-tag at N-terminus & purified by proprietarychromatographic techniques.
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