Rat Immunology ELISA Kits 2

Rat Complement C4 / C4 ELISA Kit

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SKU:
RTFI00613
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P08649
Sensitivity:
0.469ng/ml
Range:
0.781-50ng/ml
ELISA Type:
Sandwich
Synonyms:
C4
Reactivity:
Rat
Research Area:
Immunology

Description

Rat Complement C4 / C4 ELISA Kit

Complement C4, a pivotal protein within the complement system, plays a pivotal role in immune response modulation, opsonization, and clearance of immune complexes. The Rat Complement C4 ELISA Kit not only facilitates the precise quantification of Complement C4 levels but also offers invaluable insights into its dynamic interactions, empowering researchers to gain profound insights into the complex realm of immune system orchestration.

system_update_alt Datasheet system_update_alt MSDS

Key Features

Save Time Pre-coated 96 well plate
Quick Start Kit includes all necessary reagents
Publication Ready Reproducible and reliable results

Overview

Product Name:

Rat C4 (Complement Component 4) ELISA Kit

Product Code:

RTFI00613

Size:

96 Assays

Alias:

C4

Detection Method:

Sandwich ELISA, Double Antibody

Reactivity

Rat

Sensitivity:

0.469ng/ml

Range:

0.781-50ng/ml

Storage:

4°C for 6 months

Note:

For Research Use Only

Additional Information

Recovery

Matrices listed below were spiked with certain level of Rat C4 and the recovery rates were calculated by comparing the measured value to the expected amount of Rat C4 in samples.

Matrix

Recovery Range (%)

Average (%)

serum (n=5)

93-105

99

EDTA plasma (n=5)

88-102

96

UFH plasma (n=5)

86-102

96

Linearity:

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Rat C4 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample

1:2

1:4

1:8

Serum (n=5)

87-104%

88-97%

85-101%

EDTA plasma (n=5)

87-94%

83-93%

84-100%

UFH Plasma (n=5)

81-98%

85-97%

80-98%

Intra Assay

CV < 8%

Inter Assay

CV < 10%

Kit Components

Component Quantity Storage

ELISA Microplate (Dismountable)

8x12 strips

4°C for 6 months

Lyophilized Standard

2

4°C/ -20°C

Sample/Standard Dlution Buffer

20ml

4°C

Biotin-labeled Antibody (Concentrated)

120ul

4°C (Protection from light)

Antibody Dilution Buffer

10ml

4°C

HRP-Streptavidin Conjugate (SABC)

120ul

4°C (Protect from light)

SABC Dilution Buffer

10ml

4°C

TMB Substrate

10ml

4°C (Protection from light)

Stop Solution

10ml

4°C

Wash Buffer (25X)

30ml

4°C

Plate Sealer

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