Neurotrophins Recombinant Proteins

Rat CNTF Recombinant Protein (RPPB0132)

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SKU:
RPPB0132
Product Type:
Recombinant Protein
Species:
Rat
Uniprot:
P21460
Research Area:
Neurotrophins

Description

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Product Name:Rat CNTF Recombinant Protein
Product Code:RPPB0132
Size:10µg
Species:Rat
Target:CNTF
Synonyms:Ciliaryneurotrophic factor, CNTF, Cntf.
Source:Escherichia Coli
Physical Appearance:Sterilefiltered colorless solution.
Formulation:CNTF proteinsolution (0.5mg/ml) containing phosphate buffered saline (pH 7.4) and 10%glycerol.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks.Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Purity:Greaterthan 95.0% as determined by SDS-PAGE.
Amino Acid Sequence:MGSSHHHHHH SSGLVPRGSH MGSMAFAEQT PLTLHRRDLC SRSIWLARKI RSDLTALMES YVKHQGLNKNINLDSVDGVP VASTDRWSEM TEAERLQENL QAYRTFQGML TKLLEDQRVH FTPTEGDFHQ AIHTLMLQVSAFAYQLEELM VLLEQKIPEN EADGMPATVG DGGLFEKKLW GLKVLQELSQ WTVRSIHDLR VISSHQMGISALESHYGAKD KQM

CNTF is a polypeptide hormone whose actions appear to be restricted to the nervous system where it promotes neurotransmitter synthesis and neurite outgrowth in certain neuronal populations. The protein is a potent survival factor for neurons and oligodendrocytes and may be relevant in reducing tissue destruction during inflammatory attacks. A mutation in this gene, which results in aberrant splicing, leads to ciliary neurotrophic factor deficiency, but this phenotype is not causally related to neurologic disease. In addition to the predominant monocistronic transcript originating from this locus, the gene is also co-transcribed with the upstream ZFP91 gene. Co-transcription from the two loci results in a transcript that contains a complete coding region for the zinc finger protein but lacks a complete coding region for ciliary neurotrophic factor.CNTF is a survival factor for various neuronal cell types. Seems to prevent the degeneration of motor axons after axotomy.

CNTF RatRecombinant produced in E. coli is a single, non-glycosylated polypeptide chaincontaining 223 amino acids (1-200a.a) and having a molecular mass of 25.2kDa.CNTF isfused to a 23 amino acid His-tag at N-terminus & purified by proprietarychromatographic techniques.

UniProt Protein Function:CST3: As an inhibitor of cysteine proteinases, this protein is thought to serve an important physiological role as a local regulator of this enzyme activity. Defects in CST3 are the cause of amyloidosis type 6 (AMYL6); also known as hereditary cerebral hemorrhage with amyloidosis (HCHWA), cerebral amyloid angiopathy (CAA) or cerebroarterial amyloidosis Icelandic type. AMYL6 is a hereditary generalized amyloidosis due to cystatin C amyloid deposition. Cystatin C amyloid accumulates in the walls of arteries, arterioles, and sometimes capillaries and veins of the brain, and in various organs including lymphoid tissue, spleen, salivary glands, and seminal vesicles. Amyloid deposition in the cerebral vessels results in cerebral amyloid angiopathy, cerebral hemorrhage and premature stroke. Cystatin C levels in the cerebrospinal fluid are abnormally low. Genetic variations in CST3 are associated with age- related macular degeneration type 11 (ARMD11). ARMD is a multifactorial eye disease and the most common cause of irreversible vision loss in the developed world. In most patients, the disease is manifest as ophthalmoscopically visible yellowish accumulations of protein and lipid that lie beneath the retinal pigment epithelium and within an elastin-containing structure known as Bruch membrane. Belongs to the cystatin family.
UniProt Protein Details:

Protein type:Secreted, signal peptide; Secreted; Inhibitor

Cellular Component: axon; basement membrane; cell projection; cell soma; contractile fiber; cytoplasm; endoplasmic reticulum; extracellular region; extracellular space; lysosome; multivesicular body; nuclear membrane; perinuclear region of cytoplasm; vesicle

Molecular Function:beta-amyloid binding; cysteine protease inhibitor activity; endopeptidase inhibitor activity; identical protein binding; protease binding; protease inhibitor activity

Biological Process: defense response; fibril organization and biogenesis; negative regulation of peptidase activity; negative regulation of proteolysis; positive regulation of cell proliferation; positive regulation of DNA replication; regulation of programmed cell death; response to oxidative stress

NCBI Summary:The protein encoded by this gene is a cysteine protease inhibitor involved in neurodegenerative and cardiovascular processes. The encoded protein inhibits aggregation of beta-amyloid protein, a hallmark of Alzheimer's disease, so it may be useful as a therapeutic. This protein also may be a biomarker for atherosclerosis. [provided by RefSeq, Aug 2015]
UniProt Code:P21460
NCBI GenInfo Identifier:1345935
NCBI Gene ID:13010
NCBI Accession:P21460.2
UniProt Secondary Accession:P21460,Q544Y0,
UniProt Related Accession:P21460
Molecular Weight:15,531 Da
NCBI Full Name:Cystatin-C
NCBI Synonym Full Names:cystatin C
NCBI Official Symbol:Cst3��
NCBI Official Synonym Symbols:CysC��
NCBI Protein Information:cystatin-C
UniProt Protein Name:Cystatin-C
UniProt Synonym Protein Names:Cystatin-3
Protein Family:Cystatin
UniProt Gene Name:Cst3��
UniProt Entry Name:CYTC_MOUSE
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