Rat Immunology ELISA Kits 2

Rat bFGF / FGF2 / BFGF / FGF2 ELISA Kit

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SKU:
RTFI00595
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P13109
Sensitivity:
9.375pg/ml
Range:
15.625-1000pg/ml
ELISA Type:
Sandwich
Synonyms:
bFGF, FGF2, FGF-2, FGF2AS, GFG1, HBGH-2, NUDT6, Prostatropin, Basic fibroblast growth factor, basic fibroblast growth factor bFGF, FGFB, fibroblast growth factor 2, basic, HBGF-2, heparin-binding growth factor 2
Reactivity:
Rat
Research Area:
Cardiovascular

Description

Rat PDGFB ELISA Kit

Basic fibroblast growth factor (bFGF), also known as FGF2 (Fibroblast Growth Factor 2), is a protein that plays a crucial role in cell growth, development, and tissue repair.The Rat bFGF/FGF2 ELISA Kit is specifically designed for the quantitative measurement of rat basic fibroblast growth factor (bFGF/FGF2) in biological samples. The kit aids in various research applications, including studying the role of bFGF/FGF2 in development, tissue repair, angiogenesis, and disease processes.

system_update_alt Datasheet system_update_alt MSDS

Key Features

Save Time Pre-coated 96 well plate
Quick Start Kit includes all necessary reagents
Publication Ready Reproducible and reliable results

Overview

Product Name:

Rat bFGF/FGF2 (Basic Fibroblast Growth Factor) ELISA Kit

Product Code:

RTFI00595

Size:

96 Assays

Alias:

bFGF, FGF2, FGF-2, FGF2AS, GFG1, HBGH-2, NUDT6, Prostatropin, Basic fibroblast growth factor, basic fibroblast growth factor bFGF, FGFB, fibroblast growth factor 2, basic, HBGF-2, heparin-binding growth factor 2

Detection Method:

Sandwich ELISA, Double Antibody

Reactivity:

Rat

Sensitivity:

9.375pg/ml

Range:

15.625-1000pg/ml

Storage:

4°C for 6 months

Note:

For Research Use Only

Additional Information

Recovery

Matrices listed below were spiked with certain level of Rat bFGF/FGF2 and the recovery rates were calculated by comparing the measured value to the expected amount of Rat bFGF/FGF2 in samples.

Matrix

Recovery Range (%)

Average (%)

serum (n=5)

85-101

96

EDTA plasma (n=5)

86-102

93

UFH plasma (n=5)

90-102

96

Linearity:

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Rat bFGF/FGF2 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample

1:2

1:4

1:8

Serum (n=5)

86-100%

90-105%

93-102%

EDTA plasma (n=5)

90-100%

85-96%

85-99%

UFH Plasma (n=5)

80-93%

80-91%

86-94%

CV(%)

Intra Assay <8

Inter Assay <10

Kit Components

Component Quantity Storage

ELISA Microplate (Dismountable)

8x12 strips

2-8°C/-20°C

Lyophilized Standard

2

2-8°C/-20°C

Sample/Standard Dlution Buffer

20ml

2-8°C

Biotin-labeled Antibody (Concentrated)

120ul

2-8°C (Protection from light)

Antibody Dilution Buffer

10ml

2-8°C

HRP-Streptavidin Conjugate (SABC)

120ul

2-8°C(Protect from light)

SABC Dilution Buffer

10ml

2-8°C

TMB Substrate

10ml

2-8°C(Protection from light)

Stop Solution

10ml

2-8°C

Wash Buffer (25X)

30ml

2-8°C

Plate Sealer

5

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