Rat Signaling ELISA Kits 2
Rat AQP-4 (Aquaporin 4) CLIA Kit (RTES00044)
- SKU:
- RTES00044
- Product Type:
- ELISA Kit
- ELISA Type:
- CLIA Kit
- Size:
- 96 Assays
- Sensitivity:
- 18.75pg/mL
- Range:
- 31.25-2000pg/mL
- ELISA Type:
- Sandwich
- Synonyms:
- AQP4, HMIWC2, MIWC
- Reactivity:
- Rat
- Sample Type:
- Serum, plasma and other biological fluids
- Research Area:
- Signal Transduction
Description
| Assay type: | Sandwich |
| Format: | 96T |
| Assay time: | 4.5h |
| Reactivity: | Rat |
| Detection method: | Chemiluminescence |
| Detection range: | 31.25-2000 pg/mL |
| Sensitivity: | 18.75 pg/mL |
| Sample volume: | 100µL |
| Sample type: | Serum, plasma and other biological fluids |
| Repeatability: | CV < 15% |
| Specificity: | This kit recognizes Rat AQP-4 in samples. No significant cross-reactivity or interference between Rat AQP-4 and analogues was observed. |
This kit uses Sandwich-CLIA as the method. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat AQP-4. Standards or samples are added to the appropriate micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat AQP-4 and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat AQP-4, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured spectrophotometrically by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat AQP-4. The concentration of Rat AQP-4 in the samples can be calculated by comparing the RLU of the samples to the standard curve.
| UniProt Protein Function: | AQP4: a member of the aquaporin family of intrinsic membrane proteins that function as water-selective channels in the plasma membranes of many cells. The predominant aquaporin found in brain. Two alternatively spliced transcript variants have been described |
| UniProt Protein Details: | Protein type:Channel, misc. ; Membrane protein, multi-pass; Transporter, aquaporin family; Transporter; Membrane protein, integral Cellular Component: basal plasma membrane; basolateral plasma membrane; cell projection membrane; cytoplasm; external side of plasma membrane; integral to membrane; integral to plasma membrane; intercellular junction; membrane; plasma membrane; pore complex; protein complex; sarcolemma; T-tubule Molecular Function:glycerol channel activity; porin activity; transporter activity; water channel activity Biological Process: carbon dioxide transport; female pregnancy; glycerol transport; hyperosmotic salinity response; multicellular organismal water homeostasis; negative regulation of interleukin-1 beta production; negative regulation of interleukin-6 production; protein homooligomerization; response to glucocorticoid stimulus; response to radiation; sensory perception of sound; transmembrane transport; transport; water homeostasis; water transport |
| NCBI Summary: | This gene encodes a member of the aquaporin family of intrinsic membrane proteins that function as water-selective channels in the plasma membranes of many cells. This protein is the predominant aquaporin found in brain and has an important role in brain water homeostasis. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. A recent study provided evidence for translational readthrough in this gene and expression of an additional C-terminally extended isoform via the use of an alternative in-frame translation termination codon. [provided by RefSeq, Dec 2015] |
| UniProt Code: | P47863 |
| NCBI GenInfo Identifier: | 395759219 |
| NCBI Gene ID: | 25293 |
| NCBI Accession: | NP_001257487. 1 |
| UniProt Related Accession: | P47863 |
| Molecular Weight: | 28,768 Da |
| NCBI Full Name: | aquaporin-4 isoform 3 |
| NCBI Synonym Full Names: | aquaporin 4 |
| NCBI Official Symbol: | Aqp4 |
| NCBI Official Synonym Symbols: | Miwc; WCH4; AQP-4 |
| NCBI Protein Information: | aquaporin-4 |
| UniProt Protein Name: | Aquaporin-4 |
| UniProt Synonym Protein Names: | Mercurial-insensitive water channel; MIWC; WCH4 |
| Protein Family: | Aquaporin |
| UniProt Gene Name: | Aqp4 |
| UniProt Entry Name: | AQP4_RAT |
As the RLU values of the standard curve may vary according to the conditions of the actual assay performance (e. g. operator, pipetting technique, washing technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data is provided below for reference only.
| Concentration (pg/mL) | RLU | Average | Corrected |
| 2000 | 54921 56521 | 55721 | 55693 |
| 1000 | 21704 26244 | 23974 | 23946 |
| 500 | 11293 10771 | 11032 | 11004 |
| 250 | 4979 5609 | 5294 | 5266 |
| 125 | 2702 2514 | 2608 | 2580 |
| 62.5 | 1381 1241 | 1311 | 1283 |
| 31.25 | 649 699 | 674 | 646 |
| 0 | 28 28 | 28 | -- |
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Rat AQP-4 were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Rat AQP-4 were tested on 3 different plates, 20 replicates in each plate.
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (pg/mL) | 109.93 | 260.12 | 787.27 | 119.88 | 271.23 | 864.65 |
| Standard deviation | 13.21 | 19.30 | 64.01 | 12.35 | 32.36 | 75.66 |
| C V (%) | 12.02 | 7.42 | 8.13 | 10.30 | 11.93 | 8.75 |
Recovery
The recovery of Rat AQP-4 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.
| Sample Type | Range (%) | Average Recovery (%) |
| Serum (n=5) | 84-95 | 90 |
| EDTA plasma (n=5) | 85-96 | 91 |
| Cell culture media (n=5) | 99-117 | 107 |
Linearity
Samples were spiked with high concentrations of Rat AQP-4 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.
| Serum (n=5) | EDTA plasma (n=5) | Cell culture media (n=5) | ||
| 1:2 | Range (%) | 100-115 | 87-100 | 102-119 |
| Average (%) | 107 | 93 | 109 | |
| 1:4 | Range (%) | 92-107 | 93-106 | 101-114 |
| Average (%) | 100 | 99 | 107 | |
| 1:8 | Range (%) | 87-101 | 102-115 | 91-102 |
| Average (%) | 94 | 109 | 97 | |
| 1:16 | Range (%) | 86-101 | 96-110 | 97-109 |
| Average (%) | 93 | 104 | 102 |
An unopened kit can be stored at 4°C for 1 month. If the kit is not used within 1 month, store the items separately according to the following conditions once the kit is received.
| Item | Specifications | Storage |
| Micro CLIA Plate(Dismountable) | 8 wells ×12 strips | -20°C, 6 months |
| Reference Standard | 2 vials | |
| Concentrated Biotinylated Detection Ab (100×) | 1 vial, 120 µL | |
| Concentrated HRP Conjugate (100×) | 1 vial, 120 µL | -20°C(shading light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 4°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | |
| HRP Conjugate Diluent | 1 vial, 14 mL | |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | |
| Substrate Reagent A | 1 vial, 5 mL | 4°C (shading light) |
| Substrate Reagent B | 1 vial, 5 mL | 4°C (shading light) |
| Plate Sealer | 5 pieces | |
| Product Description | 1 copy | |
| Certificate of Analysis | 1 copy |
- Set standard, test sample and control (zero) wells on the pre-coated plate and record theirpositions. It is recommended to measure each standard and sample in duplicate. Note: addall solutions to the bottom of the plate wells while avoiding contact with the well walls. Ensuresolutions do not foam when adding to the wells.
- Aliquot 100µl of standard solutions into the standard wells.
- Add 100µl of Sample / Standard dilution buffer into the control (zero) well.
- Add 100µl of properly diluted sample (serum, plasma, tissue homogenates and otherbiological fluids. ) into test sample wells.
- Cover the plate with the sealer provided in the kit and incubate for 90 min at 37°C.
- Aspirate the liquid from each well, do not wash. Immediately add 100µL of BiotinylatedDetection Ab working solution to each well. Cover the plate with a plate seal and gently mix. Incubate for 1 hour at 37°C.
- Aspirate or decant the solution from the plate and add 350µL of wash buffer to each welland incubate for 1-2 minutes at room temperature. Aspirate the solution from each well andclap the plate on absorbent filter paper to dry. Repeat this process 3 times. Note: a microplatewasher can be used in this step and other wash steps.
- Add 100µL of HRP Conjugate working solution to each well. Cover with a plate seal andincubate for 30 min at 37°C.
- Aspirate or decant the solution from each well. Repeat the wash process for five times asconducted in step 7.
- Add 100µL of Substrate mixture solution to each well. Cover with a new plate seal andincubate for no more than 5 min at 37°C. Protect the plate from light.
- Determine the RLU value of each well immediately.
Related Products
Rat AQP-4 (Aquaporin 4) ELISA Kit
