Rat Agrin Recombinant Protein (RPPB2717)
- SKU:
- RPPB2717
Description
| Product Name: | Rat Agrin Recombinant Protein |
| Product Code: | RPPB2717 |
| Size: | 20µg |
| Species: | Rat |
| Target: | Agrin |
| Synonyms: | Agrin, Agrn, C90, C22, Agrin N-terminal 110 kDa subunit, Agrin C-terminal 110 kDa subunit, Agrin C-terminal 90 kDa fragment, Agrin C-terminal 22 kDa fragment, AGR |
| Source: | Sf9 Insect cells |
| Physical Appearance: | Sterile Filtered colorless solution. |
| Formulation: | The Agrin solution (0.5mg/ml) contains 10% Glycerol in Phosphate-Buffered Saline (pH 7.4). |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 90.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | ADPSCYNSPL GCCSDGKTPS LDSEGSNCPA TKAFQGVLEL EGVEGQELFY TPEMADPKSE LFGETARSIE STLDDLFRNS DVKKDFWSVR LRELGPGKLV RAIVDVHFDP TTAFQASDVG QALLRQIQVS RPWALAVRRP LQEHVRFLDF DWFPTFFTGA ATGTTAAMAT ARATTVSRLP ASSVTPRVYP SHTSRPVGRT TAPPTTRRPP TTATNMDRPR TPGHQQPSKS CDSQPCLHGGTCQDQDSGKG FTCSCTAGRG GSVCEKVQPP SMPAFKGHSF LAFPTLRAYH TLRLALEFRA LETEGLLLYN GNARGKDFLA LALLDGRVQF RFDTGSGPAV LTSLVPVEPG RWHRLELSRH WRQGTLSVDG ETPVVGESPS GTDGLNLDTN LYVGGIPEEQ VAMVLDRTSV GVGLKGCIRM LDINNQQLEL SDWQRAAVQS SGVGECGDHP CLPNPCHGGA LCQALEAGMF LCQCPPGRFG PTCADEKSPC QPNPCHGAAP CRVLSSGGAK CECPLGRSGT FCQTVLETAG SRPFLADFNG FSYLELKGLH TFERDLGEKM ALEMVFLARG PSGLLLYNGQ KTDGKGDFVS LALHNRHLEF CYDLGKGAAV IRSKEPIALG TWVRVFLERN GRKGALQVGD GPRVLGESPK SRKVPHTMLN LKEPLYIGGA PDFSKLARGA AVSSGFSGVI QLVSLRGHQL LTQEHVLRAV DVSPFADHPC TQALGNPCLN GGSCVPREAT YECLCPGGFS GLHCEKGLVE HHHHHH |
Agrin or AGRN is a large protein (proteoglycan) that has a crucial part in the development of neuromuscular junction amid embryogenesis. The protein has an involvement in the collection and aggregation of acetylcholine receptors through synaptogenesis. The agrin gene can be found and expressed in rat embryonic nervous system andmuscle tissue. This Agrin protein is aggregated in the synapses, there it can take part in regeneration & development. The protein binds to receptors on the surface of skeletal muscle.
Agrin Rat produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 766 amino acids (997-1753 a.a.) and having a molecular mass of 82.5kDa.Agrin is fused to a 9 amino acid His tag at C-Terminus and purified by proprietary chromatographic techniques.
| UniProt Protein Function: | AGRN: Plays a central role in the formation and the maintenance of the neuromuscular junction (NMJ), the synapse between motor neuron and skeletal muscle. Ligand of the MUSK signaling complex that directly binds LRP4 in this complex and induces the phosphorylation of MUSK, the kinase of the complex. The activation of MUSK in myotubes induces the formation of NMJ by regulating different processes including the transcription of specific genes and the clustering of AChR in the postsynaptic membrane. Defects in AGRN are a cause of myasthenia, limb-girdle, familial (LGM). A congenital myasthenic syndrome characterized by a typical 'limb girdle' pattern of muscle weakness with small, simplified neuromuscular junctions but normal acetylcholine receptor and acetylcholinesterase function. |
| UniProt Protein Details: | Protein type:Apoptosis; Cell adhesion; Cell development/differentiation; Extracellular matrix; Cell surface; Secreted, signal peptide; Secreted Cellular Component: basal lamina; basement membrane; cell junction; cell surface; cytoplasm; extracellular matrix; extracellular region; extracellular space; integral to membrane; plasma membrane; proteinaceous extracellular matrix; sarcolemma; synapse Molecular Function:acetylcholine receptor regulator activity; ATPase inhibitor activity; calcium ion binding; heparan sulfate proteoglycan binding; laminin binding; protein binding; sialic acid binding; transforming growth factor beta binding Biological Process: acetylcholine receptor signaling, muscarinic pathway; neuromuscular junction development; neurotransmitter receptor metabolic process; plasma membrane organization and biogenesis; positive regulation of filopodium formation; positive regulation of GTPase activity; positive regulation of neuron apoptosis; positive regulation of peptidyl-tyrosine phosphorylation; positive regulation of protein amino acid phosphorylation; positive regulation of protein binding; positive regulation of synaptic growth at neuromuscular junction; positive regulation of transcription from RNA polymerase II promoter; protein heterotetramerization; receptor clustering; regulation of GTPase activity; regulation of protein amino acid phosphorylation; regulation of receptor activity; regulation of synaptic growth at neuromuscular junction; synaptic transmission; synaptogenesis |
| NCBI Summary: | may play a role in synapse development and regeneration [RGD, Feb 2006] |
| UniProt Code: | P25304 |
| NCBI GenInfo Identifier: | 399021 |
| NCBI Gene ID: | 25592 |
| NCBI Accession: | P25304.2 |
| UniProt Secondary Accession: | P25304,Q63034, |
| UniProt Related Accession: | P25304 |
| Molecular Weight: | 208,146 Da |
| NCBI Full Name: | Agrin |
| NCBI Synonym Full Names: | agrin |
| NCBI Official Symbol: | Agrn�� |
| NCBI Official Synonym Symbols: | AGR�� |
| NCBI Protein Information: | agrin |
| UniProt Protein Name: | Agrin |
| Protein Family: | Agrin |
| UniProt Gene Name: | Agrn�� |
| UniProt Entry Name: | AGRIN_RAT |
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