RASSF3 Antibody (PACO59201)
- SKU:
- PACO59201
- Product Type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Applications:
- ELISA
- Applications:
- IHC
- Applications:
- IF
- Antibody Type:
- Polyclonal Antibody
- Conjugation:
- Unconjugated
Description
| Antibody Name: | RASSF3 Antibody (PACO59201) |
| Antibody SKU: | PACO59201 |
| Size: | 50ug |
| Host Species: | Rabbit |
| Tested Applications: | ELISA, IHC, IF |
| Recommended Dilutions: | ELISA:1:2000-1:10000, IHC:1:200-1:500, IF:1:50-1:200 |
| Species Reactivity: | Human |
| Immunogen: | Recombinant Human Ras association domain-containing protein 3 protein (2-109AA) |
| Form: | Liquid |
| Storage Buffer: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
| Purification Method: | >95%, Protein G purified |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Conjugate: | Non-conjugated |
 | IHC image of PACO59201 diluted at 1:300 and staining in paraffin-embedded human adrenal gland tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
 | Immunofluorescence staining of A549 cells with PACO59201 at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). |
| Background: | cytosol, plasma membrane, identical protein binding |
| Synonyms: | Ras association domain-containing protein 3, RASSF3 |
| UniProt Protein Function: | RASSF3: 2 isoforms of the human protein are produced by alternative splicing.Protein type: Adaptor/scaffoldChromosomal Location of Human Ortholog: 12q14.2Cellular Component: cytoplasm; plasma membraneMolecular Function: identical protein binding; protein binding |
| UniProt Protein Details: | |
| NCBI Summary: | The RAS oncogene (MIM 190020) is mutated in nearly one-third of all human cancers. Members of the RAS superfamily are plasma membrane GTP-binding proteins that modulate intracellular signal transduction pathways. A subfamily of RAS effectors, including RASSF3, share a RAS association (RA) domain.[supplied by OMIM, Jul 2003] |
| UniProt Code: | Q86WH2 |
| NCBI GenInfo Identifier: | 74714093 |
| NCBI Gene ID: | 283349 |
| NCBI Accession: | Q86WH2.1 |
| UniProt Secondary Accession: | Q86WH2,Q86WH1 |
| UniProt Related Accession: | Q86WH2 |
| Molecular Weight: | 10,265 Da |
| NCBI Full Name: | Ras association domain-containing protein 3 |
| NCBI Synonym Full Names: | Ras association domain family member 3 |
| NCBI Official Symbol: | RASSF3 |
| NCBI Official Synonym Symbols: | RASSF5 |
| NCBI Protein Information: | ras association domain-containing protein 3 |
| UniProt Protein Name: | Ras association domain-containing protein 3 |
| UniProt Synonym Protein Names: | |
| Protein Family: | Ras association domain-containing protein |
| UniProt Gene Name: | RASSF3 |
| UniProt Entry Name: | RASF3_HUMAN |
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