Description
Antibody Name: | RASL11B Antibody (PACO62199) |
Antibody SKU: | PACO62199 |
Size: | 50ul |
Host Species: | Rabbit |
Tested Applications: | ELISA, IHC, IF |
Recommended Dilutions: | ELISA:1:2000-1:10000, IHC:1:20-1:200, IF:1:50-1:200 |
Species Reactivity: | Human |
Immunogen: | Recombinant Human Ras-like protein family member 11B protein (1-248AA) |
Form: | Liquid |
Storage Buffer: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
Purification Method: | Antigen Affinity Purified |
Clonality: | Polyclonal |
Isotype: | IgG |
Conjugate: | Non-conjugated |
IHC image of PACO62199 diluted at 1:100 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. | |
Immunofluorescence staining of MCF-7 cells with PACO62199 at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). |
Synonyms: | Ras-like protein family member 11B, RASL11B |
UniProt Protein Function: | RASL11B: Belongs to the small GTPase superfamily. Ras family. Induced by TGFB1Protein type: G protein, monomeric, Ras; G protein; G protein, monomericChromosomal Location of Human Ortholog: 4q12 |
UniProt Protein Details: | |
NCBI Summary: | RASL11B is a member of the small GTPase protein family with a high degree of similarity to RAS (see HRAS, MIM 190020) proteins.[supplied by OMIM, Nov 2008] |
UniProt Code: | Q9BPW5 |
NCBI GenInfo Identifier: | 74732795 |
NCBI Gene ID: | 65997 |
NCBI Accession: | Q9BPW5.1 |
UniProt Secondary Accession: | Q9BPW5,Q96KC5, B2RC51 |
UniProt Related Accession: | Q9BPW5 |
Molecular Weight: | 27,508 Da |
NCBI Full Name: | Ras-like protein family member 11B |
NCBI Synonym Full Names: | RAS like family 11 member B |
NCBI Official Symbol: | RASL11B |
NCBI Official Synonym Symbols: | |
NCBI Protein Information: | ras-like protein family member 11B |
UniProt Protein Name: | Ras-like protein family member 11B |
UniProt Synonym Protein Names: | |
Protein Family: | Ras-like protein family |
UniProt Gene Name: | RASL11B |
UniProt Entry Name: | RSLBB_HUMAN |