Rabbit IGF1 Recombinant Protein (RPPB0427)

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SKU:
RPPB0427
Product Type:
Recombinant Protein
Species:
Rabbit
Uniprot:
Q95222

Description

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Product Name:Rabbit IGF1 Recombinant Protein
Product Code:RPPB0427
Size:50µg
Species:Rabbit
Target:IGF1
Synonyms:Somatomedin C, IGF-I, IGFI, IGF1, IGF-IA, Mechano growth factor, MGF.
Source:Escherichia Coli
Physical Appearance:Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation:The protein was lyophilized from a concentrated (0.5mg/ml) solution with 0.02% NaHCO3.
Solubility:It is recommended to reconstitute the lyophilized rbIGF-I in sterile 0.4% NaHCO3 adjusted to ph 8-9, not less than 100�g/ml, which can then be further diluted to other aqueous solutions.
Stability:Lyophilized Insulin-Like Growth Factor-1 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IGF1 should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.
Purity:Greater than 98.0% �as determined by:(a) Gel-filtration chromatography under non denaturing conditions.(b) Analysis by SDS-PAGE.
Amino Acid Sequence:MTAGPETLCG AELVDALQFV CGDRGFYFNK PTGYGSSSRR APQTGIVDEC CFRSCDLRRL EMYCAPLKP
Biological Activity:IGF-I is biologically active when compared to human IGF-1. The ED50, calculated by the dose -dependent proliferation of human MCF/7 cells is 5 to 25ng/ml in the cell culture mixture dependent on culture conditions. Its activity consists of 30-40 % compared to human IGF-1.

The IGFs comprise a family of peptides that play important roles in mammalian growth and development. IGF1 mediates many of the growth-promoting effects of growth hormone. Early studies showed that growth hormone did not directly stimulate the incorporation of sulfate into cartilage, but rather acted through a serum factor, termed 'sulfation factor,' which later became known as 'somatomedin'. Three main somatomedins have been characterized: somatomedin C (IGF1), somatomedin A, and somatomedin B. IGF-1 is a small protein secreted mostly but not exclusively by the liver and circulating in blood mostly as a complex with several IGF binding proteins. It has growth-regulating, insulin-like, and mitogenic functions and it is secreted in response to growth hormone stimulation.

Insulin-Like Growth Factor-I Rabbit Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 71 amino acids and having a molecular mass of 7639 Dalton.�

UniProt Protein Function:Function: The insulin-like growth factors, isolated from plasma, are structurally and functionally related to insulin but have a much higher growth-promoting activity. May be a physiological regulator of [1-14C]-2-deoxy-D-glucose (2DG) transport and glycogen synthesis in osteoblasts. Stimulates glucose transport in rat bone-derived osteoblastic (PyMS) cells and is effective at much lower concentrations than insulin, not only regarding glycogen and DNA synthesis but also with regard to enhancing glucose uptake. May play a role in synapse maturation
UniProt Code:Q95222
NCBI GenInfo Identifier:126722801
NCBI Gene ID:100008668
NCBI Accession:NP_001075495.1
UniProt Secondary Accession:Q95222,O18846,
UniProt Related Accession:Q95222
Molecular Weight:16,092 Da
NCBI Full Name:insulin-like growth factor I
NCBI Official Symbol:IGF1��
NCBI Official Synonym Symbols:IGF-IB��
NCBI Protein Information:insulin-like growth factor I; IGF-I; somatomedin; insulin-like growth factor IB
UniProt Protein Name:Insulin-like growth factor I
UniProt Synonym Protein Names:Somatomedin
Protein Family:Insulin-like growth factor
UniProt Gene Name:IGF1��
UniProt Entry Name:IGF1_RABIT
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