QuickStep Human IgM (Immunoglobulin M) ELISA Kit
- SKU:
- AEES00638
- Product Type:
- ELISA Kit
- ELISA Type:
- Sandwich
- Size:
- 96 Assays
- Reactivity:
- Human
- Sensitivity:
- 1.02 ng/mL
- Range:
- 7.80-500 ng/mL
- Sample Type:
- Serum
Description
| Product Name: | QuickStep Human IgM (Immunoglobulin M) ELISA Kit |
| Product Code: | AEES00638 |
| Assay Type: | Sandwich |
| Format: | 96 Assays |
| Assay Time: | 2.5h |
| Reactivity: | Human |
| Detection Range: | 7.80-500 ng/mL |
| Sensitivity: | 1.02 ng/mL |
| Sample Type & Sample Volume: | Serum, plasma, urine, saliva, 50μL |
| Specificity: | This kit recognizes Human IgM in samples. No significant or interference between Human IgM and analogues was observed. |
| Reproducibility: | Both intra-CV and inter-CV are < 10%. |
| Application: | This ELISA kit applies to the in vitro quantitative determination of Human IgM concentrations in serum, plasma, urine, saliva.Please consult technical support for the applicability if other biological fluids need to be tested. |
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human IgM. Samples (or Standards) and biotinylated detection antibody specific for Human IgM are added to the micro ELISA plate wells. Human IgM would combine with the specific antibody. Then Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human IgM, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 ± 2 nm. The OD value is proportional to the concentration of Human IgM. You can calculate the concentration of Human IgM in the samples by comparing the OD of the samples to the standard curve.
| Kit Components: | An unopened kit can be stored at 2-8℃ for six months. After test, the unused wells and reagents should be stored according to the table below.
|
| 1. | Add 50μL standard or sample to the wells, immediately add 50μL Biotinylated Detection Ab working solution to each well. Incubate for 90 min at 37°C |
| 2. | Aspirate and wash the plate for 3 times |
| 3. | Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times |
| 4. | Add 90μL Substrate Reagent. Incubate for 15 min at 37°C |
| 5. | Add 50μL Stop Solution |
| 6. | Read the plate at 450nm immediately. Calculation of the results |
Immunoglobulin M (IgM) is a class of immunoglobulins found in blood and lymph fluid that are the first type of antibody made in response to an infection [1]. IgM antibodies make up about 5 percent to 10 percent of all the antibodies in the body. IgM is principally concerned with the primary antibody response, appearing soon after initial invasion by an antigen and capable of destroying the antigen when it is first introduced. Its normal mean of serum concentration is 120 mg/dl. IgM is the first immunoglobulin expressed in the human fetus (around 20 weeks) and phylogenetically the earliest antibody to develop [2]. IgM antibodies appear early in the course of an infection and usually reappear, to a lesser extent, after further exposure. IgM antibodies do not pass across the human placenta (only isotype IgG). The biological properties of IgM make it useful in the diagnosis of infectious diseases. Demonstrating IgM antibodies in a patient's serum indicates recent infection, o
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