QTRT1 Antibody (PACO42130)
- SKU:
- PACO42130
- Product Type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Applications:
- ELISA
- Applications:
- WB
- Applications:
- IHC
- Antibody Type:
- Polyclonal Antibody
- Conjugation:
- Unconjugated
Description
| Antibody Name: | QTRT1 Antibody (PACO42130) |
| Antibody SKU: | PACO42130 |
| Size: | 50ug |
| Host Species: | Rabbit |
| Tested Applications: | ELISA, WB, IHC |
| Recommended Dilutions: | ELISA:1:2000-1:10000, WB:1:500-1:2000, IHC:1:20-1:200 |
| Species Reactivity: | Human |
| Immunogen: | Recombinant Human Queuine tRNA-ribosyltransferase catalytic subunit 1 protein (211-403AA) |
| Form: | Liquid |
| Storage Buffer: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
| Purification Method: | >95%, Protein G purified |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Conjugate: | Non-conjugated |
 | Western blot. All lanes: QTRT1 antibody at 6µg/ml. Lane 1: MCF-7 whole cell lysate. Lane 2: PC-3 whole cell lysate. Lane 3: Jurkat whole cell lysate. Lane 4: A431 whole cell lysate. Secondary. Goat polyclonal to rabbit IgG at 1/10000 dilution. Predicted band size: 45, 25 kDa. Observed band size: 45 kDa. |
 | Immunohistochemistry of paraffin-embedded human testis tissue using PACO42130 at dilution of 1:100. |
| Background: | Catalytic subunit of the queuine tRNA-ribosyltransferase (TGT) that catalyzes the base-exchange of a guanine (G) residue with queuine (Q) at position 34 (anticodon wobble position) in tRNAs with GUN anticodons (tRNA-Asp, -Asn, -His and -Tyr), resulting in the hypermodified nucleoside queuosine (7-(((4,5-cis-dihydroxy-2-cyclopenten-1-yl)amino)methyl)-7-deazaguanosine). Catalysis occurs through a double-displacement mechanism. The nucleophile active site attacks the C1' of nucleotide 34 to detach the guanine base from the RNA, forming a covalent enzyme-RNA intermediate. The proton acceptor active site deprotonates the incoming queuine, allowing a nucleophilic attack on the C1' of the ribose to form the product (By similarity). |
| Synonyms: | Queuine tRNA-ribosyltransferase catalytic subunit 1 (EC 2.4.2.29) (Guanine insertion enzyme) (tRNA-guanine transglycosylase), QTRT1, TGT TGUT |
| UniProt Protein Function: | QTRT1: Interacts with QTRTD1 to form an active queuine tRNA- ribosyltransferase. This enzyme exchanges queuine for the guanine at the wobble position of tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr), thereby forming the hypermodified nucleoside queuosine (Q) (7-(((4,5-cis-dihydroxy-2-cyclopenten-1- yl)amino)methyl)-7-deazaguanosine). Belongs to the queuine tRNA-ribosyltransferase family.Protein type: Mitochondrial; RNA processing; EC 2.4.2.29; TransferaseChromosomal Location of Human Ortholog: 19p13.3Cellular Component: cytoplasm; mitochondrial outer membraneMolecular Function: queuine tRNA-ribosyltransferase activityBiological Process: queuosine biosynthetic process; tRNA modification |
| UniProt Protein Details: | |
| NCBI Summary: | This gene encodes the catalytic subunit of tRNA-guanine transglycosylase. tRNA-guanine transglycosylase is a heterodimeric enzyme complex that plays a critical role in tRNA modification by synthesizing the 7-deazaguanosine queuosine, which is found in tRNAs that code for asparagine, aspartic acid, histidine and tyrosine. A pseudogene of this gene is located on the long arm of chromosome X. [provided by RefSeq, Feb 2012] |
| UniProt Code: | Q9BXR0 |
| NCBI GenInfo Identifier: | 239938842 |
| NCBI Gene ID: | 81890 |
| NCBI Accession: | Q9BXR0.3 |
| UniProt Secondary Accession: | Q9BXR0,Q96BQ4, Q9BXQ9, B4DFM7 |
| UniProt Related Accession: | Q9BXR0 |
| Molecular Weight: | 24,015 Da |
| NCBI Full Name: | Queuine tRNA-ribosyltransferase |
| NCBI Synonym Full Names: | queuine tRNA-ribosyltransferase catalytic subunit 1 |
| NCBI Official Symbol: | QTRT1 |
| NCBI Official Synonym Symbols: | TGT; TGUT; FP3235 |
| NCBI Protein Information: | queuine tRNA-ribosyltransferase |
| UniProt Protein Name: | Queuine tRNA-ribosyltransferase |
| UniProt Synonym Protein Names: | Guanine insertion enzyme; tRNA-guanine transglycosylase |
| Protein Family: | Queuine tRNA-ribosyltransferase |
| UniProt Gene Name: | QTRT1 |
| UniProt Entry Name: | TGT_HUMAN |
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