Signaling Pathway Assays
Pyruvate Kinase Activity Assay Kit (BA0145)
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- SKU:
- BA0145
- Product Type:
- Assay
- Instrument:
- Microplate Reader
- Sample Type:
- Plasma, Serum, Cell, Tissue
- Research Area:
- TCA Cycle
- Research Area:
- Diabetes & Obesity
- Research Area:
- Plant & Environmental Stress
- Research Area:
- Enzyme Activity
Description
Pyruvate Kinase Activity Assay
Pyruvate Kinase (PK) is an enzyme involved in glycolysis. It catalyzes the transfer of a phosphate group from phosphoenolpyruvate (PEP) to ADP, yielding one molecule of pyruvate and one molecule of ATP. Pyruvate kinase deficiency, a genetic disease, is caused by a lack of pyruvate kinase and slows down the process of glycolysis. Pyruvate kinase is also involved in gluconeogenesis, a biochemical pathway in which the liver generates glucose from pyruvate and other substrates.
Assay Genie's Pyruvate Kinase Activity Assay Kit provides a simple, direct and automation-ready procedure for measuring pyruvate kinase activity. In this assay PEP and ADP are catalyzed by pyruvate kinase to generate pyruvate and ATP. The color intensity of the reaction product at 570nm or fluorescence intensity at Ex/Em = 530/590nm is directly proportional to the pyruvate generated by the Pyruvate Kinase in the sample.Applications
For quantitative determination of pyruvate kinase activity and evaluation of drug effects on PK activity.
Key Features
- Sensitive and accurate. Linear detection range in 96-well plate: 0.1 to 50 U/L for colorimetric assays and 0.01 to 2 U/L for fluorimetric assays run at 25°C for 30 min.
Pyruvate Kinase Activity Assay Information
| New Column | New Column |
| Kit Includes: | Developer: 12 mL, Cosubstrate: 120 mL, Dye Reagent: 120 mL, Pyruvate Standard: 400 mL 25 mM |
| Kit Requires: | Pipetting devices, clear or black flat-bottom 96-well plates, plate reader and centrifuge tubes. |
| Method of Detection: | OD570nm, FL530/585nm |
| Detection Limit: | 0.01 U/L |
| Samples: | Plasma, serum, cell and tissue etc |
| Species: | All |
| Protocol Length: | 40 min |
| Size: | 100 tests |
| Storage: | Store all kit components at -20 °C. |
| Shelf Life: | 6 months |
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