Pyruvate Decarboxylase (PDC) Activity Assay Kit
- SKU:
- MAES0260
- Product Type:
- Assay
- Instrument:
- Microplate Reader
- Detection Method:
- Colorimetric
- Sample Type:
- Serum, Plasma, Tissue And Cell Samples
- Sensitivity:
- 0.67 U/L
- Range:
- 0.67-27.73 U/L
- Assay Time:
- 15 min
Description
| Product Name: | Pyruvate Decarboxylase (PDC) Activity Assay Kit |
| Product Code: | MAES0260 |
| Sample Type: | Serum, Plasma, Tissue And Cell Samples |
| Assay Time: | 15 min |
| Assay Type: | Enzyme Activity |
| Synonyms: | PDC |
| Detection Instrument: | Microplate reader (330-350 nm, optimum wavelength: 340 nm) |
| Detection Method: | Colorimetric method |
| Sensitivity: | 0.67 U/L |
| Detection Range: | 0.67-27.73 U/L |
| Inter-assay CV: | 0.031 |
| Intra-assay CV: | 0.028 |
Pyruvate decarboxylase (PDC) mainly exists in yeast and is one of the key enzymes in ethanol fermentation. PDC catalyzes pyruvate decarboxylate to acetaldehyde, which reacts under the action of ethanol dehydrogenase (ADH), and catalyzes NADH to convert into NAD+. NADH has a characteristic absorption peak at 340 nm. The PDC activity can be calculated by measuring the OD value at 340 nm.
| Storage: | -20°C |
| Valid Period: | 12 months |
| Other Reagents Required: | Normal saline (0.9% NaCl) |
| Other Instruments Required: | Vortex mixer, Centrifuge |
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