pvdA Antibody (PACO50646)
- SKU:
- PACO50646
- Product Type:
- Antibody
- Reactivity:
- Bacteria
- Host Species:
- Rabbit
- Isotype:
- IgG
- Applications:
- ELISA
- Applications:
- WB
- Antibody Type:
- Polyclonal Antibody
- Conjugation:
- Unconjugated
Description
| Antibody Name: | pvdA Antibody (PACO50646) |
| Antibody SKU: | PACO50646 |
| Size: | 50ug |
| Host Species: | Rabbit |
| Tested Applications: | ELISA, WB |
| Recommended Dilutions: | ELISA:1:2000-1:10000, WB:1:500-1:5000 |
| Species Reactivity: | Pseudomonas aeruginosa |
| Immunogen: | Recombinant Pseudomonas aeruginosa L-ornithine N(5)-monooxygenase protein (1-443AA) |
| Form: | Liquid |
| Storage Buffer: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
| Purification Method: | >95%, Protein G purified |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Conjugate: | Non-conjugated |
 | Western Blot. Positive WB detected in Recombinant protein. All lanes: pvdA antibody at 2.8µg/ml. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 65 kDa. Observed band size: 65 kDa.. |
| Background: | Catalyzes the conversion of L-ornithine to N(5)-hydroxyornithine, the first step in the biosynthesis of all hydroxamate-containing siderophores, such as pyoverdin. Pyoverdin is a hydroxamate siderophore composed of a 6,7-dihydroxyquinoline-containing fluorescent chromophore joined to the N-terminus of a partly cyclic octapeptide (D-Ser-L-Arg-D-Ser-L-N(5)-OH-Orn-L-Lys-L-N(5)-OH-Orn-L-Thr-L-Thr in strain PAO1). Specific for NADPH, which plays a role in stabilization of the C4a-hydroperoxyflavin intermediate. |
| Synonyms: | L-ornithine N(5)-monooxygenase (EC 1.14.13.195) (L-ornithine N(5)-hydroxylase) (Ornithine hydroxylase) (L-ornithine N(5)-oxygenase) (Pyoverdin biosynthesis protein A), pvdA, pvd-1 |
| UniProt Protein Function: | Catalyzes the conversion of L-ornithine to N(5)-hydroxyornithine, the first step in the biosynthesis of all hydroxamate-containing siderophores, such as pyoverdin. Pyoverdin is a hydroxamate siderophore composed of a 6,7-dihydroxyquinoline-containing fluorescent chromophore joined to the N-terminus of a partly cyclic octapeptide (D-Ser-L-Arg-D-Ser-L-N(5)-OH-Orn-L-Lys-L-N(5)-OH-Orn-L-Thr-L-Thr in strain PAO1). Specific for NADPH, which plays a role in stabilization of the C4a-hydroperoxyflavin intermediate. |
| UniProt Protein Details: | |
| NCBI Summary: | |
| UniProt Code: | Q51548 |
| NCBI GenInfo Identifier: | 15597582 |
| NCBI Gene ID: | 882167 |
| NCBI Accession: | NP_251076.1 |
| UniProt Secondary Accession: | Q51548 |
| UniProt Related Accession: | Q51548 |
| Molecular Weight: | 65.5 kDa |
| NCBI Full Name: | L-ornithine N5-oxygenase |
| NCBI Synonym Full Names: | |
| NCBI Official Symbol: | pvdA |
| NCBI Official Synonym Symbols: | |
| NCBI Protein Information: | L-ornithine N5-oxygenase |
| UniProt Protein Name: | L-ornithine N(5)-monooxygenase |
| UniProt Synonym Protein Names: | L-ornithine N(5)-hydroxylase |
| Protein Family: | L-ornithine N(5)-monooxygenase |
| UniProt Gene Name: | pvdA |
| UniProt Entry Name: | PVDA_PSEAE |
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