Puromycin Polyclonal Antibody
- SKU:
- CAB21205
- Product Type:
- Antibody
- Antibody Type:
- Polyclonal Antibody
- Host Species:
- Rabbit
- Isotype:
- IgG
- Applications:
- WB
Description
| Product Name: | Puromycin Polyclonal Antibody |
| Product Code: | CAB21205 |
| Reactivity: | Species independent |
| Applications: | Western blotting |
| Host Species: | Rabbit |
| Purification Method: | Affinity purification |
| Isotype: | IgG |
| Reactivity: | Species independent |
| Tested Applications: | WB, ELISA |
| Key Applications: | Western blotting |
| Recommended Dilution: | WB 1:1000-1:5000 |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3. |
| Positive Samples: | 293F, RAW264.7 |
| Customer Validation: | IF(Rattus norvegicus)WB(Mus musculus) |
Puromycin is a tyrosyl-tRNA mimic that blocks translation by labeling and releasing elongating polypeptide chains from translating ribosomes. Puromycin has been used in molecular biology research for decades as a translation inhibitor.More recently, in vivo puromycylation assays have become popular tools for localizing translating ribosomes in cells. These assays often use elongation inhibitors to purportedly inhibit the release of puromycin-labeled nascent peptides from ribosomes.
| Immunogen: | Chemical compounds corresponding to Puromycin. |
| Sequence: | Email for sequence |
| Observed MW: | 10-250kDa |
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