Puromycin Monoclonal Antibody
- SKU:
- CAB23031
- Product Type:
- Antibody
- Antibody Type:
- Monoclonal Antibody
- Host Species:
- Rabbit
- Isotype:
- IgG
- Applications:
- WB
- Applications:
- IHC
- Applications:
- IF
- Applications:
- IP
Description
| Product Name: | Puromycin Monoclonal Antibody |
| Product Code: | CAB23031 |
| Reactivity: | Species independent |
| Applications: | Western blotting, Immunohistochemistry, Immunofluorescence, Immunoprecipitation |
| Host Species: | Rabbit |
| Purification Method: | Affinity purification |
| Isotype: | IgG |
| Clone No: | ARC58626 |
| Reactivity: | Species independent |
| Tested Applications: | WB, IHC-P, IF/ICC, IP, FC, FC(Intra), ELISA |
| Key Applications: | Western blotting, Immunohistochemistry, Immunofluorescence, Immunoprecipitation |
| Recommended Dilution: | WB 1:2000-1:12000, IHC-P 1:100-1:500, IF/ICC 1:50-1:200, IP 1:2000-1:6000, FC(Intra) 1:500-1:1000 |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3. |
| Positive Samples: | 293F cells, RAW264.7 cells and 293T cells were treated by puromycin |
| Customer Validation: | Puro-PLA assay(Homo sapiens) |
Puromycin is an aminonucleoside antibiotic, derived from the Streptomyces alboniger bacterium, that causes premature chain termination during translation taking place in the ribosome. It has a role as a nucleoside antibiotic, an antiinfective agent, an antineoplastic agent, a protein synthesis inhibitor, an antimicrobial agent, an EC 3.4.11.14 (cytosol alanyl aminopeptidase) inhibitor and an EC 3.4.14.2 (dipeptidyl-peptidase II) inhibitor. It is a conjugate base of a puromycin(1+).Puromycin is an antibiotic that prevents bacterial protein translation. It is utilized as a selective agent in laboratory cell cultures. Puromycin is toxic to both prokaryotic and eukaryotic cells, resulting in significant cell death at appropriate doses.
| Immunogen: | Chemical compounds corresponding to puromycin. |
| Sequence: | Email for sequence |
| Observed MW: | 10-100kDa |
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