PTCH2 Antibody (PACO56262)
- SKU:
- PACO56262
- Product Type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Applications:
- ELISA
- Applications:
- IHC
- Applications:
- IF
- Antibody Type:
- Polyclonal Antibody
- Conjugation:
- Unconjugated
Description
| Antibody Name: | PTCH2 Antibody (PACO56262) |
| Antibody SKU: | PACO56262 |
| Size: | 50ug |
| Host Species: | Rabbit |
| Tested Applications: | ELISA, IHC, IF |
| Recommended Dilutions: | ELISA:1:2000-1:10000, IHC:1:200-1:500, IF:1:50-1:200 |
| Species Reactivity: | Human |
| Immunogen: | Recombinant Human Protein patched homolog 2 protein (793-951AA) |
| Form: | Liquid |
| Storage Buffer: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
| Purification Method: | >95%, Protein G purified |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Conjugate: | Non-conjugated |
 | IHC image of PACO56262 diluted at 1:200 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
 | Immunofluorescence staining of 293 cells with PACO56262 at 1:66, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). |
| Background: | May have a role in epidermal development. May act as a receptor for Sonic hedgehog (SHH). |
| Synonyms: | Protein patched homolog 2 (PTC2), PTCH2 |
| UniProt Protein Function: | PTCH2: May have a role in epidermal development. May act as a receptor for Sonic hedgehog (SHH). Belongs to the patched family. 2 isoforms of the human protein are produced by alternative splicing.Protein type: Membrane protein, integral; Membrane protein, multi-passChromosomal Location of Human Ortholog: 1p34.1Cellular Component: integral to membraneMolecular Function: hedgehog receptor activity; smoothened bindingBiological Process: skin development; epidermis development; negative regulation of smoothened signaling pathway; signal transduction; hair cycleDisease: Basal Cell Nevus Syndrome; Basal Cell Carcinoma, Susceptibility To, 1; Medulloblastoma |
| UniProt Protein Details: | |
| NCBI Summary: | This gene encodes a transmembrane receptor of the patched gene family. The encoded protein may function as a tumor suppressor in the hedgehog signaling pathway. Alterations in this gene have been associated with nevoid basal cell carcinoma syndrome, basal cell carcinoma, medulloblastoma, and susceptibility to congenital macrostomia. Alternatively spliced transcript variants have been described.[provided by RefSeq, Oct 2009] |
| UniProt Code: | Q9Y6C5 |
| NCBI GenInfo Identifier: | 12643356 |
| NCBI Gene ID: | 8643 |
| NCBI Accession: | Q9Y6C5.2 |
| UniProt Secondary Accession: | Q9Y6C5 |
| UniProt Related Accession: | Q9Y6C5 |
| Molecular Weight: | 130kDa |
| NCBI Full Name: | Protein patched homolog 2 |
| NCBI Synonym Full Names: | patched 2 |
| NCBI Official Symbol: | PTCH2 |
| NCBI Official Synonym Symbols: | PTC2 |
| NCBI Protein Information: | protein patched homolog 2 |
| UniProt Protein Name: | Protein patched homolog 2 |
| UniProt Synonym Protein Names: | |
| Protein Family: | |
| UniProt Gene Name: | PTCH2 |
| UniProt Entry Name: | PTC2_HUMAN |
| Antibodies |
| PTCH2 Antibody (PACO11568) |
| Secondary Antibody |
| Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014) |
| Recommended Products |
| Anti-FITC Goat Anti-Rabbit IgG (H+L) Antibody (CABS011) |
| Anti-HRP-conjugated Beta Actin Antibody (CABC028) |
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