PTBP3 Antibody (PACO61975)
- SKU:
- PACO61975
- Product Type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Applications:
- ELISA
- Applications:
- WB
- Applications:
- IHC
- Applications:
- IP
- Antibody Type:
- Polyclonal Antibody
- Conjugation:
- Unconjugated
Description
| Antibody Name: | PTBP3 Antibody (PACO61975) |
| Antibody SKU: | PACO61975 |
| Size: | 50ul |
| Host Species: | Rabbit |
| Tested Applications: | ELISA, WB, IHC, IP |
| Recommended Dilutions: | ELISA:1:2000-1:10000, WB:1:1000-1:5000, IHC:1:20-1:200, IP:1:200-1:2000 |
| Species Reactivity: | Human |
| Immunogen: | Peptide sequence from Human Polypyrimidine tract-binding protein 3 protein (37-55AA) |
| Form: | Liquid |
| Storage Buffer: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
| Purification Method: | Antigen Affinity Purified |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Conjugate: | Non-conjugated |
 | Western Blot. Positive WB detected in: K562 whole cell lysate, MCF-7 whole cell lysate. All lanes: PTBP3 antibody at 1:2000. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 60, 57, 61, 50 kDa. Observed band size: 60 kDa. |
 | Immunoprecipitating PTBP3 in A549 whole cell lysate. Lane 1: Rabbit control IgG instead of PACO61975 in A549 whole cell lysate.For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/50000). Lane 2: PACO61975 (5µg) + A549 whole cell lysate (0.5mg). Lane 3: A549 whole cell lysate (20µg). |
 | IHC image of PACO61975 diluted at 1:100 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
| Background: | RNA-binding protein that mediates pre-mRNA alternative splicing regulation. Plays a role in the regulation of cell proliferation, differentiation and migration. Positive regulator of EPO-dependent erythropoiesis. Participates in cell differentiation regulation by repressing tissue-specific exons. Promotes FAS exon 6 skipping. Binds RNA, preferentially to both poly(G) and poly(U). |
| Synonyms: | Polypyrimidine tract-binding protein 3 (Regulator of differentiation 1) (Rod1), PTBP3, ROD1 |
| UniProt Protein Function: | ROD1: RNA-binding protein that mediates pre-mRNA alternative splicing regulation. Plays a role in the regulation of cell proliferation, differentiation and migration. Positive regulator of EPO-dependent erythropoiesis. Participates in cell differentiation regulation by repressing tissue-specific exons. Promotes FAS exon 6 skipping. Binds RNA, preferentially to both poly(G) and poly(U). 6 isoforms of the human protein are produced by alternative splicing.Protein type: RNA-bindingChromosomal Location of Human Ortholog: 9q32Molecular Function: RNA bindingBiological Process: anatomical structure morphogenesis; negative regulation of RNA splicing |
| UniProt Protein Details: | |
| NCBI Summary: | The protein encoded by this gene binds RNA and is a regulator of cell differentiation. The encoded protein preferentially binds to poly(G) and poly(U) sequences in vitro. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2011] |
| UniProt Code: | O95758 |
| NCBI GenInfo Identifier: | 218511814 |
| NCBI Gene ID: | 9991 |
| NCBI Accession: | O95758.2 |
| UniProt Secondary Accession: | O95758,Q68DB9, Q86YB3, Q86YH9, B1ALY2, B1ALY3, B1ALY5 B1ALY6, B3KME7 |
| UniProt Related Accession: | O95758 |
| Molecular Weight: | 49,540 Da |
| NCBI Full Name: | Polypyrimidine tract-binding protein 3 |
| NCBI Synonym Full Names: | polypyrimidine tract binding protein 3 |
| NCBI Official Symbol: | PTBP3 |
| NCBI Official Synonym Symbols: | ROD1 |
| NCBI Protein Information: | polypyrimidine tract-binding protein 3 |
| UniProt Protein Name: | Polypyrimidine tract-binding protein 3 |
| UniProt Synonym Protein Names: | Regulator of differentiation 1; Rod1 |
| Protein Family: | Polypyrimidine tract-binding protein |
| UniProt Gene Name: | PTBP3 |
| UniProt Entry Name: | PTBP3_HUMAN |
| Antibodies |
| PTBP3 Antibody (PACO11886) |
| Secondary Antibody |
| Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014) |
| Recommended Products |
| Anti-FITC Goat Anti-Rabbit IgG (H+L) Antibody (CABS011) |
| Anti-HRP-conjugated Beta Actin Antibody (CABC028) |
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