Name: PTAFR Antibody (PACO29248)
SKU: PACO29248
Availability: OutOfStock

Antibody Name:	PTAFR Antibody (PACO29248)
Antibody SKU:	PACO29248
Size:	50ug
Host Species:	Rabbit
Tested Applications:	ELISA, WB, IHC, IF
Recommended Dilutions:	ELISA:1:2000-1:10000, WB:1:500-1:2000, IHC:1:20-1:200, IF:1:50-1:200
Species Reactivity:	Human, Mouse
Immunogen:	Recombinant Human Platelet-activating factor receptor protein (297-342AA)

Form:	Liquid
Storage Buffer:	Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Purification Method:	>95%, Protein G purified
Clonality:	Polyclonal
Isotype:	IgG
Conjugate:	Non-conjugated

	Western blot All lanes: PTAFR antibody at 4µg/ml + Mouse lung tissue Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 40 kDa Observed band size: 40 kDa .
	Immunofluorescent analysis of A431 cells using PACO29248 at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
	Immunohistochemistry of paraffin-embedded human kidney tissue using PACO29248 at dilution of 1:100.

Background:	Receptor for platelet activating factor, a chemotactic phospholipid mediator that possesses potent inflammatory, smooth-muscle contractile and hypotensive activity. Seems to mediate its action via a G protein that activates a phosphatidylinositol-calcium second messenger system.
Synonyms:	Platelet-activating factor receptor (PAF-R) (PAFr), PTAFR, PAFR

UniProt Protein Function:	PTAFR: Receptor for platelet activating factor, a chemotactic phospholipid mediator that possesses potent inflammatory, smooth- muscle contractile and hypotensive activity. Seems to mediate its action via a G protein that activates a phosphatidylinositol- calcium second messenger system. Belongs to the G-protein coupled receptor 1 family.Protein type: Membrane protein, integral; GPCR, family 1; Membrane protein, multi-pass; Motility/polarity/chemotaxis; Receptor, GPCRChromosomal Location of Human Ortholog: 1p35-p34.3Cellular Component: nucleoplasm; membrane; intracellular membrane-bound organelle; integral to plasma membrane; integral to membrane; plasma membraneMolecular Function: G-protein coupled receptor activity; platelet activating factor receptor activity; purinergic nucleotide receptor activity, G-protein coupled; lipopolysaccharide binding; phospholipid binding; lipopolysaccharide receptor activityBiological Process: G-protein coupled receptor protein signaling pathway; phosphoinositide-mediated signaling; inositol trisphosphate biosynthetic process; cytokine and chemokine mediated signaling pathway; lipopolysaccharide-mediated signaling pathway; cytokine production; immune response; inflammatory response; chemotaxis
UniProt Protein Details:
NCBI Summary:	This gene encodes a seven-transmembrane G-protein-coupled receptor for platelet-activating factor (PAF) that localizes to lipid rafts and/or caveolae in the cell membrane. PAF (1-0-alkyl-2-acetyl-sn-glycero-3-phosphorylcholine) is a phospholipid that plays a significant role in oncogenic transformation, tumor growth, angiogenesis, metastasis, and pro-inflammatory processes. Binding of PAF to the PAF-receptor (PAFR) stimulates numerous signal transduction pathways including phospholipase C, D, A2, mitogen-activated protein kinases (MAPKs), and the phosphatidylinositol-calcium second messenger system. Following PAFR activation, cells become rapidly desensitized and this refractory state is dependent on PAFR phosphorylation, internalization, and down-regulation. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2011]
UniProt Code:	P25105
NCBI GenInfo Identifier:	257900493
NCBI Gene ID:	5724
NCBI Accession:	NP_001158195.1
UniProt Secondary Accession:	P25105,A3KMC8, A8K2H5
UniProt Related Accession:	P25105
Molecular Weight:	39,203 Da
NCBI Full Name:	platelet-activating factor receptor
NCBI Synonym Full Names:	platelet-activating factor receptor
NCBI Official Symbol:	PTAFR
NCBI Official Synonym Symbols:	PAFR
NCBI Protein Information:	platelet-activating factor receptor; PAF-R
UniProt Protein Name:	Platelet-activating factor receptor
UniProt Synonym Protein Names:
Protein Family:
UniProt Gene Name:	PTAFR
UniProt Entry Name:	PTAFR_HUMAN