PRUNE1 Antibody (PACO37362)
- SKU:
- PACO37362
- Product Type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Applications:
- ELISA
- Applications:
- WB
- Applications:
- IHC
- Applications:
- IF
- Applications:
- IP
- Antibody Type:
- Polyclonal Antibody
- Conjugation:
- Unconjugated
Description
| Antibody Name: | PRUNE1 Antibody (PACO37362) |
| Antibody SKU: | PACO37362 |
| Size: | 50ug |
| Host Species: | Rabbit |
| Tested Applications: | ELISA, WB, IHC, IF, IP |
| Recommended Dilutions: | ELISA:1:2000-1:10000, WB:1:1000-1:5000, IHC:1:200-1:500, IF:1:50-1:200, IP:1:200-1:2000, |
| Species Reactivity: | Human |
| Immunogen: | Recombinant Human Exopolyphosphatase PRUNE1 protein (1-168AA) |
| Form: | Liquid |
| Storage Buffer: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
| Purification Method: | >95%, Protein G purified |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Conjugate: | Non-conjugated |
 | Western Blot. Positive WB detected in: HepG2 whole cell lysate, MCF-7 whole cell lysate, Jurkat whole cell lysate. All lanes: PRUNE1 antibody at 4µg/ml. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 51, 43, 31, 27, 25, 19 kDa. Observed band size: 60 kDa. |
 | Immunofluorescence staining of HepG2 cells with PACO37362 at 1:135, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). |
 | IHC image of PACO37362 diluted at 1:300 and staining in paraffin-embedded human lung tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
| Background: | Phosphodiesterase (PDE) that has higher activity toward cAMP than cGMP, as substrate. Plays a role in cell proliferation, migration and differentiation, and acts as a negative regulator of NME1. Plays a role in the regulation of neurogenesis. Involved in the regulation of microtubule polymerization. |
| Synonyms: | Exopolyphosphatase PRUNE1 (EC 3.6.1.1) (Drosophila-related expressed sequence 17) (DRES-17) (DRES17) (HTcD37) (Protein prune homolog 1) (hPrune), PRUNE1, PRUNE |
| UniProt Protein Function: | PRUNE: Phosphodiesterase (PDE) that has higher activity toward cAMP than cGMP, as substrate. Plays a role in cell proliferation, is able to induce cell motility and acts as a negative regulator of NME1. Belongs to the PPase class C family. Prune subfamily. 7 isoforms of the human protein are produced by alternative splicing.Protein type: Hydrolase; EC 3.6.1.1; Nucleotide Metabolism - purineChromosomal Location of Human Ortholog: 1q21Cellular Component: cytoplasmMolecular Function: protein binding |
| UniProt Protein Details: | |
| NCBI Summary: | This gene encodes a member of the DHH protein superfamily of phosphoesterases. This protein has been found to function as both a nucleotide phosphodiesterase and an exopolyphosphatase. This protein is believed to stimulate cancer progression and metastases through the induction of cell motility. A pseuodgene has been identified on chromosome 13. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2014] |
| UniProt Code: | Q86TP1 |
| NCBI GenInfo Identifier: | 229462737 |
| NCBI Gene ID: | 58497 |
| NCBI Accession: | Q86TP1.2 |
| UniProt Secondary Accession: | Q86TP1,Q5SZF9, Q659E5, Q6P4E0, Q8N654, Q96JU5, Q9C071 Q9C072, Q9UIV0, B2RCH8 |
| UniProt Related Accession: | Q86TP1 |
| Molecular Weight: | 24,475 Da |
| NCBI Full Name: | Protein prune homolog |
| NCBI Synonym Full Names: | prune exopolyphosphatase |
| NCBI Official Symbol: | PRUNE1 |
| NCBI Official Synonym Symbols: | PRUNE; DRES17; HTCD37; DRES-17; H-PRUNE |
| NCBI Protein Information: | protein prune homolog |
| UniProt Protein Name: | Protein prune homolog |
| UniProt Synonym Protein Names: | Drosophila-related expressed sequence 17; DRES-17; DRES17; HTcD37 |
| Protein Family: | Protein prune |
| UniProt Gene Name: | PRUNE |
| UniProt Entry Name: | PRUNE_HUMAN |
| Antibodies |
| PRUNE Antibody (PACO11512) |
| Secondary Antibody |
| Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014) |
| Recommended Products |
| Anti-FITC Goat Anti-Rabbit IgG (H+L) Antibody (CABS011) |
| Anti-HRP-conjugated Beta Actin Antibody (CABC028) |
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