Name: PRPF40A Antibody (PACO61582)
SKU: PACO61582
Availability: OutOfStock

Antibody Name:	PRPF40A Antibody (PACO61582)
Antibody SKU:	PACO61582
Size:	50ug
Host Species:	Rabbit
Tested Applications:	ELISA, WB, IHC
Recommended Dilutions:	ELISA:1:2000-1:10000, WB:1:500-1:5000, IHC:1:200-1:500
Species Reactivity:	Human
Immunogen:	Recombinant Human Pre-mRNA-processing factor 40 homolog A protein (120-266AA)

Form:	Liquid
Storage Buffer:	Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Purification Method:	>95%, Protein G purified
Clonality:	Polyclonal
Isotype:	IgG
Conjugate:	Non-conjugated

Western Blot. Positive WB detected in: U251 whole cell lysate, Hela whole cell lysate, 293T whole cell lysate. All lanes: PRPF40A antibody at 2.7µg/ml. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 109, 106, 107, 11 kDa. Observed band size: 109 kDa.

IHC image of PACO61582 diluted at 1:200 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Background:	Binds to WASL/N-WASP and suppresses its translocation from the nucleus to the cytoplasm, thereby inhibiting its cytoplasmic function (By similarity). Plays a role in the regulation of cell morphology and cytoskeletal organization. Required in the control of cell shape and migration. May play a role in cytokinesis. May be involved in pre-mRNA splicing.
Synonyms:	Pre-mRNA-processing factor 40 homolog A, Fas ligand-associated factor 1, Formin-binding protein 11, Formin-binding protein 3, Huntingtin yeast partner A, Huntingtin-interacting protein 10, HIP-10, Huntingtin-interacting protein A, Renal carcinoma antigen NY-REN-6, PRPF40A, FBP11, FLAF1, FNBP3, HIP10, HYPA, HSPC225

UniProt Protein Function:	FNBP3: Binds to WASL/N-WASP and suppresses its translocation from the nucleus to the cytoplasm, thereby inhibiting its cytoplasmic function. Plays a role in the regulation of cell morphology and cytoskeletal organization. Required in the control of cell shape and migration. May play a role in cytokinesis. May be involved in pre-mRNA splicing. Belongs to the PRPF40 family. 4 isoforms of the human protein are produced by alternative splicing.Protein type: RNA processing; SpliceosomeChromosomal Location of Human Ortholog: 2q23.3Cellular Component: cytoplasm; membrane; nuclear matrix; nucleoplasm; snRNP U1Molecular Function: protein binding; RNA bindingBiological Process: cell migration; cytoskeleton organization and biogenesis; nuclear mRNA splicing, via spliceosome; regulation of cell shape; regulation of cytokinesis
UniProt Protein Details:
NCBI Summary:
UniProt Code:	O75400
NCBI GenInfo Identifier:	3341990
NCBI Gene ID:	55660
NCBI Accession:	AAC27506.1
UniProt Secondary Accession:	O75400,O43856, O75404, Q8TBQ1, Q9H782, Q9NWU9, Q9P0Q2 Q9Y5A8
UniProt Related Accession:	O75400
Molecular Weight:	10,697 Da
NCBI Full Name:	huntingtin-interacting protein HYPA/FBP11, partial
NCBI Synonym Full Names:	pre-mRNA processing factor 40 homolog A
NCBI Official Symbol:	PRPF40A
NCBI Official Synonym Symbols:	HYPA; FBP11; FLAF1; FNBP3; HIP10; Prp40; FBP-11; HIP-10; NY-REN-6
NCBI Protein Information:	pre-mRNA-processing factor 40 homolog A
UniProt Protein Name:	Pre-mRNA-processing factor 40 homolog A
UniProt Synonym Protein Names:	Fas ligand-associated factor 1; Formin-binding protein 11; Formin-binding protein 3; Huntingtin yeast partner A; Huntingtin-interacting protein 10; HIP-10; Huntingtin-interacting protein A; Renal carcinoma antigen NY-REN-6
Protein Family:	Hydrophobin
UniProt Gene Name:	PRPF40A
UniProt Entry Name: