PRPF39 Antibody (PACO61638)
- SKU:
- PACO61638
- Product Type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Applications:
- ELISA
- Applications:
- IHC
- Applications:
- IF
- Antibody Type:
- Polyclonal Antibody
- Conjugation:
- Unconjugated
Description
| Antibody Name: | PRPF39 Antibody (PACO61638) |
| Antibody SKU: | PACO61638 |
| Size: | 50ug |
| Host Species: | Rabbit |
| Tested Applications: | ELISA, IHC, IF |
| Recommended Dilutions: | ELISA:1:2000-1:10000, IHC:1:500-1:1000, IF:1:50-1:200 |
| Species Reactivity: | Human |
| Immunogen: | Recombinant Human Pre-mRNA-processing factor 39 protein (41-190AA) |
| Form: | Liquid |
| Storage Buffer: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
| Purification Method: | >95%, Protein G purified |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Conjugate: | Non-conjugated |
 | IHC image of PACO61638 diluted at 1:500 and staining in paraffin-embedded human glioma performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
 | Immunofluorescence staining of U251 cells with PACO61638 at 1:166, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). |
 | IHC image of PACO61638 diluted at 1:500 and staining in paraffin-embedded human liver cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
| Background: | Involved in pre-mRNA splicing. |
| Synonyms: | Pre-mRNA-processing factor 39, PRP39 homolog, PRPF39 |
| UniProt Protein Function: | PRPF39: Involved in pre-mRNA splicing. Belongs to the PRP39 family. 2 isoforms of the human protein are produced by alternative splicing.Protein type: RNA processingChromosomal Location of Human Ortholog: 14q21.2Cellular Component: snRNP U1Molecular Function: pre-mRNA 5'-splice site bindingBiological Process: nuclear mRNA 5'-splice site recognition |
| UniProt Protein Details: | |
| NCBI Summary: | |
| UniProt Code: | Q86UA1 |
| NCBI GenInfo Identifier: | 223590245 |
| NCBI Gene ID: | 55015 |
| NCBI Accession: | Q86UA1.3 |
| UniProt Secondary Accession: | Q86UA1,Q08AL1, Q08AL2, Q9NUU5 |
| UniProt Related Accession: | Q86UA1 |
| Molecular Weight: | 31,494 Da |
| NCBI Full Name: | Pre-mRNA-processing factor 39 |
| NCBI Synonym Full Names: | pre-mRNA processing factor 39 |
| NCBI Official Symbol: | PRPF39 |
| NCBI Official Synonym Symbols: | |
| NCBI Protein Information: | pre-mRNA-processing factor 39 |
| UniProt Protein Name: | Pre-mRNA-processing factor 39 |
| UniProt Synonym Protein Names: | PRP39 homolog |
| Protein Family: | |
| UniProt Gene Name: | PRPF39 |
| UniProt Entry Name: | PRP39_HUMAN |
| Antibodies |
| PRPF39 Antibody (PACO04670) |
| Secondary Antibody |
| Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014) |
| Recommended Products |
| Anti-FITC Goat Anti-Rabbit IgG (H+L) Antibody (CABS011) |
| Anti-HRP-conjugated Beta Actin Antibody (CABC028) |
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