Protein Carbonyl Assay Kit - Colorimetric (MAES0096)
- SKU:
- MAES0096
- Product Type:
- Assay
- Detection Method:
- Colorimetric
- Instrument:
- Microplate Reader
- Sample Type:
- Serum, Plasma, Hydrothorax, Cell Culture Supernatant, Tissue
- Assay Time:
- 140 min
- Shipping:
- Gel Pack
- Research Area:
- Oxidative Stress
- Research Area:
- Antioxidant
Description
system_update_altDatasheet
| Product Name: | Protein Carbonyl Assay Kit - Colorimetric |
| Product Code: | MAES0096 |
| Product Size: | 96 Assays |
| Sample Type: | Serum, plasma, hydrothorax, cell culture supernatant, tissue |
| Assay Time: | 140 min |
| Instrument: | Microplate reader |
| Recovery Rate: | 97 |
| Inter CV: | 8.5 |
| Inter CV: | 5.2 |
The reactive oxygen species produced by aerobic metabolism in the body can cause the oxidation of DNA, lipids and proteins. The secondary reaction of the amino acid side chain of the protein with the lipid oxidation product is the main cause of the formation of the carbonyl. Carbonyl is a biological marker of ROS-mediated protein oxidation.
The content of protein carbonyl increases after oxidation, and the carbonyl group reacts with 2, 4-dinitrophenylhydrazine to form a reddish brown precipitate. The absorbance can be measured at 370 nm after the precipitation is dissolved. The carbonyl content can be calculated indirectly.
| Shelf Life: | 6 Months |
| Storage: | 2-8°C |
| Shipping: | Gel Pack |
| Research Area: | Oxidative stress, Amino acids & proteins |
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