Antibody Name:	PREX2 Antibody (PACO47946)
Antibody SKU:	PACO47946
Size:	50ug
Host Species:	Rabbit
Tested Applications:	ELISA, WB, IHC, IF
Recommended Dilutions:	ELISA:1:2000-1:10000, WB:1:2000-1:5000, IHC:1:20-1:200, IF:1:50-1:200
Species Reactivity:	Human, Mouse
Immunogen:	Recombinant Human Phosphatidylinositol 3,4,5-trisphosphate-dependent Rac exchanger 2 protein (1417-1592AA)

Form:	Liquid
Storage Buffer:	Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Purification Method:	>95%, Protein G purified
Clonality:	Polyclonal
Isotype:	IgG
Conjugate:	Non-conjugated

	Western Blot. Positive WB detected in: Mouse brain tissue. All lanes: PREX2 antibody at 3.4µg/ml. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 183, 172, 113 kDa. Observed band size: 183, 113 kDa..
	Immunofluorescent analysis of HepG2 cells using PACO47946 at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
	Immunohistochemistry of paraffin-embedded human kidney tissue using PACO47946 at dilution of 1:100.

Background:

Functions as a RAC1 guanine nucleotide exchange factor (GEF), activating Rac proteins by exchanging bound GDP for free GTP. Its activity is synergistically activated by phosphatidylinositol 3,4,5-trisphosphate and the β γ subunits of heterotrimeric G protein. Mediates the activation of RAC1 in a PI3K-dependent manner. May be an important mediator of Rac signaling, acting directly downstream of both G protein-coupled receptors and phosphoinositide 3-kinase.

Synonyms:

Phosphatidylinositol 3,4,5-trisphosphate-dependent Rac exchanger 2 protein (P-Rex2) (PtdIns(3,4,5)-dependent Rac exchanger 2) (DEP domain-containing protein 2), PREX2, DEPDC2

UniProt Protein Function:	PREX2: Functions as a RAC1 guanine nucleotide exchange factor (GEF), activating Rac proteins by exchanging bound GDP for free GTP. Its activity is synergistically activated by phosphatidylinositol 3,4,5-trisphosphate and the beta gamma subunits of heterotrimeric G protein. Mediates the activation of RAC1 in a PI3K-dependent manner. May be an important mediator of Rac signaling, acting directly downstream of both G protein- coupled receptors and phosphoinositide 3-kinase. 3 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:GEFs; GEFs, Rac/Rho; Phosphatase, regulatory subunit Chromosomal Location of Human Ortholog: 8q13.2 Cellular Component: cytoplasm; plasma membrane Molecular Function:GTPase activator activity; Rac guanyl-nucleotide exchange factor activity Biological Process: G-protein coupled receptor protein signaling pathway; negative regulation of protein kinase activity; negative regulation of TOR signaling pathway
NCBI Summary:	The protein encoded by this gene belongs to the phosphatidylinositol 3,4,5-trisphosphate (PIP3)-dependent Rac exchanger (PREX) family, which are Dbl-type guanine-nucleotide exchange factors for Rac family small G proteins. Structural domains of this protein include the catalytic diffuse B-cell lymphoma homology and pleckstrin homology (DHPH) domain, two disheveled, EGL-10, and pleckstrin homology (DEP) domains, two PDZ domains, and a C-terminal inositol polyphosphate-4 phosphatase (IP4P) domain that is found in one of the isoforms. This protein facilitates the exchange of GDP for GTP on Rac1, allowing the GTP-bound Rac1 to activate downstream effectors. Studies also show that the pleckstrin homology domain of this protein interacts with the phosphatase and tensin homolog (PTEN) gene product to inhibit PTEN phosphatase activity, thus activating the phosphoinositide-3 kinase (PI3K) signaling pathway. Conversely, the PTEN gene product has also been shown to inhibit the GEF activity of this protein. This gene plays a role in insulin-signaling pathways, and either mutations or overexpression of this gene have been observed in some cancers. [provided by RefSeq, Apr 2016]
UniProt Code:	Q70Z35
NCBI GenInfo Identifier:	47578115
NCBI Gene ID:	80243
NCBI Accession:	NP_079146.2
UniProt Secondary Accession:	Q70Z35,Q32KL0, Q32KL1, Q6R7Q3, Q6R7Q4, Q9H805, Q9H961 B4DFX0,
UniProt Related Accession:	Q70Z35
Molecular Weight:
NCBI Full Name:	phosphatidylinositol 3,4,5-trisphosphate-dependent Rac exchanger 2 protein isoform a
NCBI Synonym Full Names:	phosphatidylinositol-3,4,5-trisphosphate dependent Rac exchange factor 2
NCBI Official Symbol:	PREX2
NCBI Official Synonym Symbols:	DEP.2; DEPDC2; P-REX2; PPP1R129
NCBI Protein Information:	phosphatidylinositol 3,4,5-trisphosphate-dependent Rac exchanger 2 protein
UniProt Protein Name:	Phosphatidylinositol 3,4,5-trisphosphate-dependent Rac exchanger 2 protein
UniProt Synonym Protein Names:	DEP domain-containing protein 2
Protein Family:	Phosphatidylinositol 3,4,5-trisphosphate-dependent Rac exchanger 2 protein
UniProt Gene Name:	PREX2
UniProt Entry Name:	PREX2_HUMAN