PRAMEF18 Antibody (PACO62343)
- SKU:
- PACO62343
- Product Type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Applications:
- ELISA
- Applications:
- WB
- Applications:
- IHC
- Antibody Type:
- Polyclonal Antibody
- Conjugation:
- Unconjugated
Description
| Antibody Name: | PRAMEF18 Antibody (PACO62343) |
| Antibody SKU: | PACO62343 |
| Size: | 50ul |
| Host Species: | Rabbit |
| Tested Applications: | ELISA, WB, IHC |
| Recommended Dilutions: | ELISA:1:2000-1:10000, WB:1:1000-1:5000, IHC:1:200-1:500 |
| Species Reactivity: | Human |
| Immunogen: | Recombinant Human PRAME family member 18 protein (1-257AA) |
| Form: | Liquid |
| Storage Buffer: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
| Purification Method: | >95%, Protein G purified |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Conjugate: | Non-conjugated |
 | Western Blot. Positive WB detected in: HepG2 whole cell lysate, Hela whole cell lysate, MCF-7 whole cell lysate. All lanes: PRAMEF18 antibody at 1:2000. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 56 kDa. Observed band size: 56 kDa. |
 | IHC image of PACO62343 diluted at 1:400 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
| Synonyms: | PRAME family member 18, PRAMEF18 |
| UniProt Protein Function: | PRAMEF18: Belongs to the PRAME family.Chromosomal Location of Human Ortholog: 1p36.21 |
| UniProt Protein Details: | |
| NCBI Summary: | |
| UniProt Code: | Q5VWM3 |
| NCBI GenInfo Identifier: | 978128207 |
| NCBI Gene ID: | 391003 |
| NCBI Accession: | Q5VWM3.2 |
| UniProt Secondary Accession: | Q5VWM3 |
| UniProt Related Accession: | Q5VWM3 |
| Molecular Weight: | 55,334 Da |
| NCBI Full Name: | PRAME family member 18 |
| NCBI Synonym Full Names: | PRAME family member 18 |
| NCBI Official Symbol: | PRAMEF18 |
| NCBI Official Synonym Symbols: | |
| NCBI Protein Information: | PRAME family member 18 |
| UniProt Protein Name: | PRAME family member 18 |
| UniProt Synonym Protein Names: | |
| Protein Family: | PRAME family |
| UniProt Gene Name: | PRAMEF18 |
| UniProt Entry Name: | PRA18_HUMAN |
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