PPCDC Antibody (PACO59860)
- SKU:
- PACO59860
- Product Type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Applications:
- ELISA
- Applications:
- IHC
- Applications:
- IF
- Antibody Type:
- Polyclonal Antibody
- Conjugation:
- Unconjugated
Description
| Antibody Name: | PPCDC Antibody (PACO59860) |
| Antibody SKU: | PACO59860 |
| Size: | 50ug |
| Host Species: | Rabbit |
| Tested Applications: | ELISA, IHC, IF |
| Recommended Dilutions: | ELISA:1:2000-1:10000, IHC:1:500-1:1000, IF:1:50-1:200 |
| Species Reactivity: | Human |
| Immunogen: | Recombinant Human Phosphopantothenoylcysteine decarboxylase protein (51-183AA) |
| Form: | Liquid |
| Storage Buffer: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
| Purification Method: | >95%, Protein G purified |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Conjugate: | Non-conjugated |
 | IHC image of PACO59860 diluted at 1:500 and staining in paraffin-embedded human endometrial cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
 | Immunofluorescence staining of A549 cells with PACO59860 at 1:166, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). |
| Background: | Necessary for the biosynthesis of coenzyme A. Catalyzes the decarboxylation of 4-phosphopantothenoylcysteine to form 4'-phosphopantotheine. |
| Synonyms: | Phosphopantothenoylcysteine decarboxylase (PPC-DC) (EC 4.1.1.36) (CoaC), PPCDC, COAC |
| UniProt Protein Function: | PPCDC: Necessary for the biosynthesis of coenzyme A. Catalyzes the decarboxylation of 4-phosphopantothenoylcysteine to form 4'- phosphopantotheine. Belongs to the HFCD (homooligomeric flavin containing Cys decarboxylase) superfamily. 2 isoforms of the human protein are produced by alternative splicing.Protein type: EC 4.1.1.36; Lyase; Cofactor and Vitamin Metabolism - pantothenate and CoA biosynthesisChromosomal Location of Human Ortholog: 15q24.2Cellular Component: cytosolMolecular Function: phosphopantothenoylcysteine decarboxylase activity; protein bindingBiological Process: coenzyme A biosynthetic process; coenzyme biosynthetic process |
| UniProt Protein Details: | |
| NCBI Summary: | Biosynthesis of coenzyme A (CoA) from pantothenic acid (vitamin B5) is an essential universal pathway in prokaryotes and eukaryotes. PPCDC (EC 4.1.1.36), one of the last enzymes in this pathway, converts phosphopantothenoylcysteine to 4-prime-phosphopantetheine (Daugherty et al., 2002 [PubMed 11923312]).[supplied by OMIM, Mar 2008] |
| UniProt Code: | Q96CD2 |
| NCBI GenInfo Identifier: | 296434457 |
| NCBI Gene ID: | 60490 |
| NCBI Accession: | Q96CD2.2 |
| UniProt Secondary Accession: | Q96CD2,Q96SX0, Q9HC17 |
| UniProt Related Accession: | Q96CD2 |
| Molecular Weight: | 14,069 Da |
| NCBI Full Name: | Phosphopantothenoylcysteine decarboxylase |
| NCBI Synonym Full Names: | phosphopantothenoylcysteine decarboxylase |
| NCBI Official Symbol: | PPCDC |
| NCBI Official Synonym Symbols: | coaC; MDS018; PPC-DC |
| NCBI Protein Information: | phosphopantothenoylcysteine decarboxylase |
| UniProt Protein Name: | Phosphopantothenoylcysteine decarboxylase |
| UniProt Synonym Protein Names: | CoaC |
| Protein Family: | Putative phosphopantothenoylcysteine decarboxylase |
| UniProt Gene Name: | PPCDC |
| UniProt Entry Name: | COAC_HUMAN |
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