Name: Porcine T Cell Response Array (19 targets) (SARB0098)
SKU: SARB0098
Availability: OutOfStock

Product Sku:	SARB0098
Size:	2, 4, or 8
Species Detected:	Porcine
Number of Targets Detected:	19
Gene Symbols:	CSF2, IFNG, IL10, IL12A, IL13, IL15, IL17, IL17A, IL17F, IL1A\|IL1B, IL1RN, IL21, IL22, IL28B, IL4, IL6, IRAP1, NCAM1, TGFB1, TNF, TNFA, TNFSF2
Compatible Sample Types:	Cell Culture Supernatants, Cell Lysates, Plasma, Serum, Tissue Lysates
Design Principle:	Sandwich-based
Method of Detection:	Chemiluminescence
Pathway:
Quantitative/Semi-Quantitative:	Semi-Quantitative
Solid Support:	Membrane
Suggested Application(s):	Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Storage/Stability:	For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.

Hover over target for more information
GM-CSF Gene Symbol: CSF2 Gene ID: 397208 Accession #: Q29118	IFN-gamma Gene Symbol: IFNG Gene ID: 396991 Accession #: P17803	IL-1 alpha (IL-1 F1) Gene Symbol: IL1A Gene ID: 397094 Accession #: P18430	IL-1 beta (IL-1 F2) Gene Symbol: IL1B Gene ID: 397122 Accession #: P26889	IL-1 Ra (IL-1 F3) Gene Symbol: IL1RN IRAP1 Gene ID: 397499 Accession #: Q29056
IL-10 Gene Symbol: IL10 Gene ID: 397106 Accession #: Q29055	IL-12 p70 Gene Symbol: IL12A Gene ID: 397053 Accession #: Q29053	IL-13 Gene Symbol: IL13 Gene ID: 396721 Accession #: Q95J68	IL-15 Gene Symbol: IL15 Gene ID: 397683 Accession #: Q95253	IL-17A Gene Symbol: IL17A IL17 Gene ID: 449530 Accession #: Q60I29
IL-17F Gene Symbol: IL17F Gene ID: N/A Accession #: F1RKV0	IL-21 Gene Symbol: IL21 Gene ID: 403123 Accession #: Q76LU6	IL-22 Gene Symbol: IL22 Gene ID: 595104 Accession #: K7GM74	IL-28B Gene Symbol: IL28B Gene ID: 100310828 Accession #: D0FHA1	IL-4 Gene Symbol: IL4 Gene ID: 397225 Accession #: Q04745
IL-6 Gene Symbol: IL6 Gene ID: 100628202; 399500 Accession #: P26893	NCAM-1 (CD56) Gene Symbol: NCAM1 Gene ID: N/A Accession #: F1SM72	TGF beta 1 Gene Symbol: TGFB1 Gene ID: 397078 Accession #: P07200	TNF alpha Gene Symbol: TNF TNFA TNFSF2 Gene ID: 397086 Accession #: P23563

Pipettors, pipet tips and other common lab consumables
Orbital shaker or oscillating rocker
Tissue Paper, blotting paper or chromatography paper
Adhesive tape or Saran Wrap
Distilled or de-ionized water
A chemiluminescent blot documentation system , X-ray Film and a suitable film processor, or another chemiluminescent detection system.

1.	Block membranes
2.	Incubate with Sample
3.	Incubate with Biotinylated Detection Antibody Cocktail
4.	Incubate with HRP-Conjugated Streptavidin
5.	Incubate with Detection Buffers
6.	Image with chemiluminescent imaging system
7.	Perform densitometry and analysis

Porcine T Cell Response Array (19 targets) (SARB0098)

Description