Porcine T Cell Response Array (19 targets) (SARB0098)

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SKU:
SARB0098
Product Type:
Protein Array
Analytes:
GM-CSF
Analytes:
IFN-gamma
Analytes:
IL-1 alpha (IL-1 F1)
Analytes:
IL-1 beta (IL-1 F2)
Analytes:
IL-1 Ra (IL-1 F3)
Analytes:
IL-10
Analytes:
IL-12 p70
Analytes:
IL-13
Analytes:
IL-15
Analytes:
IL-17A
Analytes:
IL-17F
Analytes:
IL-21
Analytes:
IL-22
Analytes:
IL-28B
Analytes:
IL-4
Analytes:
IL-6
Analytes:
NCAM-1 (CD56)
Analytes:
TGF beta 1
Analytes:
TNF alpha
Reactivity:
Porcine
Applications:
Multiplex Array

Description

system_update_altDatasheet
Product Sku:SARB0098
Size:2, 4, or 8
Species Detected:Porcine
Number of Targets Detected:19
Gene Symbols:CSF2, IFNG, IL10, IL12A, IL13, IL15, IL17, IL17A, IL17F, IL1A|IL1B, IL1RN, IL21, IL22, IL28B, IL4, IL6, IRAP1, NCAM1, TGFB1, TNF, TNFA, TNFSF2
Compatible Sample Types:Cell Culture Supernatants, Cell Lysates, Plasma, Serum, Tissue Lysates
Design Principle:Sandwich-based
Method of Detection:Chemiluminescence
Pathway:
Quantitative/Semi-Quantitative:Semi-Quantitative
Solid Support:Membrane
Suggested Application(s):Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Storage/Stability:For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period.
Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
Hover over target for more information
GM-CSF
  • Gene Symbol: CSF2
  • Gene ID: 397208
  • Accession #: Q29118
IFN-gamma
  • Gene Symbol: IFNG
  • Gene ID: 396991
  • Accession #: P17803
IL-1 alpha (IL-1 F1)
  • Gene Symbol: IL1A
  • Gene ID: 397094
  • Accession #: P18430
IL-1 beta (IL-1 F2)
  • Gene Symbol: IL1B
  • Gene ID: 397122
  • Accession #: P26889
IL-1 Ra (IL-1 F3)
  • Gene Symbol: IL1RN IRAP1
  • Gene ID: 397499
  • Accession #: Q29056
IL-10
  • Gene Symbol: IL10
  • Gene ID: 397106
  • Accession #: Q29055
IL-12 p70
  • Gene Symbol: IL12A
  • Gene ID: 397053
  • Accession #: Q29053
IL-13
  • Gene Symbol: IL13
  • Gene ID: 396721
  • Accession #: Q95J68
IL-15
  • Gene Symbol: IL15
  • Gene ID: 397683
  • Accession #: Q95253
IL-17A
  • Gene Symbol: IL17A IL17
  • Gene ID: 449530
  • Accession #: Q60I29
IL-17F
  • Gene Symbol: IL17F
  • Gene ID: N/A
  • Accession #: F1RKV0
IL-21
  • Gene Symbol: IL21
  • Gene ID: 403123
  • Accession #: Q76LU6
IL-22
  • Gene Symbol: IL22
  • Gene ID: 595104
  • Accession #: K7GM74
IL-28B
  • Gene Symbol: IL28B
  • Gene ID: 100310828
  • Accession #: D0FHA1
IL-4
  • Gene Symbol: IL4
  • Gene ID: 397225
  • Accession #: Q04745
IL-6
  • Gene Symbol: IL6
  • Gene ID: 100628202; 399500
  • Accession #: P26893
NCAM-1 (CD56)
  • Gene Symbol: NCAM1
  • Gene ID: N/A
  • Accession #: F1SM72
TGF beta 1
  • Gene Symbol: TGFB1
  • Gene ID: 397078
  • Accession #: P07200
TNF alpha
  • Gene Symbol: TNF TNFA TNFSF2
  • Gene ID: 397086
  • Accession #: P23563
  • Easy to use
  • No specialized equipment needed
  • Compatible with nearly any liquid sample
  • Proven technology
  • Highly sensitive (pg/ml)
  • Sandwich ELISA specificity
  • Higher density than ELISA, Western blot or bead-based multiplex
  • Porcine T Cell Response Antibody Array C1 Membranes
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated HRP
  • Detection Buffer C
  • Detection Buffer D
  • Lysis Buffer
  • 8-Well Incubation Tray
  • Plastic Sheets
  • Array Templates

Other Materials Required

  • Pipettors, pipet tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Tissue Paper, blotting paper or chromatography paper
  • Adhesive tape or Saran Wrap
  • Distilled or de-ionized water
  • A chemiluminescent blot documentation system , X-ray Film and a suitable film processor, or another chemiluminescent detection system.
1.Block membranes
2.Incubate with Sample
3.Incubate with Biotinylated Detection Antibody Cocktail
4.Incubate with HRP-Conjugated Streptavidin
5.Incubate with Detection Buffers
6.Image with chemiluminescent imaging system
7.Perform densitometry and analysis
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